Method for preparing gamma-linolenic acid-rich grease through sea squirt-associated Penicillium citrinum Asc2-4 fermentation

A technology of Penicillium citrinum and linolenic acid is applied in the field of preparing oil rich in γ-linolenic acid, can solve the problems of high cost, poor stability, complicated production process and the like, and achieves the effects of tenacious vitality, low cost and simple process

Active Publication Date: 2016-10-26
GUANGDONG OCEAN UNIVERSITY
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, commercially available polyunsaturated fatty acids, especially ω-3 polyunsaturated fatty acids, are mainly extracted from deep-sea fish oil. Higher plants and animals rarely contain ω-3 polyunsaturated fatty acids. The production process is complicated, the cost is high, and the stability is poor. are also limited by climatic conditions and resources

Method used

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  • Method for preparing gamma-linolenic acid-rich grease through sea squirt-associated Penicillium citrinum Asc2-4 fermentation
  • Method for preparing gamma-linolenic acid-rich grease through sea squirt-associated Penicillium citrinum Asc2-4 fermentation

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Effect test

Embodiment 1

[0059] Example 1 Isolation and identification of ascidian symbiotic fungus Penicillium citrinum Asc2-4

[0060] 1. Strain isolation

[0061] (1) Sample: sea squirt, collected from quicksand shrimp pond in Leizhou City, Guangdong Province.

[0062] Take 10 g of sea squirt viscera, homogenize it with a homogenizer, put it into 90 mL of sterile sea water, shake and mix well. Then use sterile seawater to press 10 -2 ~10 -6 Dilute and spread on solid medium, culture at 28°C for 24-72 hours, and isolate single colonies.

[0063] The above-mentioned single colony was further inoculated into a liquid seed medium, cultured on a shaker at 28°C for 72 hours, and the fat particles in the mycelia were observed under a microscope. Suction filter and dry the fermented mycelium, extract the oil in the mycelium by acid-heat method, measure the content of gamma-linolenic acid in the oil with a gas chromatograph, and select the strain with the highest gamma-linolenic acid content as the bact...

Embodiment 2

[0074] Example 2 Preparation of gamma-linolenic acid oil by fermentation of ascidian symbiotic fungus Penicillium citrinum Asc2-4

[0075] 1. A method for preparing gamma-linolenic acid oil by fermenting the ascidian symbiotic fungus Penicillium citrinum Asc2-4, comprising the following steps:

[0076] (1) Strain activation (solid strain culture):

[0077] Transfer the strains preserved in freeze-dried tubes or sand tubes to potato dextrose agar slant medium, and culture them at 25°C for 5 days to activate the strains;

[0078] (2) Seed liquid preparation (liquid strain culture):

[0079] The slant strains were made into spore suspension and inoculated in the seed medium for cultivation. The seed medium was potato dextrose medium, the inoculation amount was 3%, the pH was 5.5-6.5, the cultivation temperature was 28°C, and the cultivation time was 2 days ;

[0080] (3) Fermentation culture

[0081] 1) Fermentation medium preparation: Accurately weigh potatoes according to t...

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Abstract

The invention discloses a method for preparing gamma-linolenic acid-rich grease through sea squirt-associated Penicillium citrinum Asc2-4 fermentation. The method comprises inoculating a fermentation medium with sea squirt-associated Penicillium citrinum Asc2-4, carrying out fermentation under the oxygen condition to obtain Penicillium citrinum mycelia, and extracting gamma-linolenic acid-rich grease from the Penicillium citrinum mycelia. The sea squirt-associated Penicillium citrinum Asc2-4 is preserved in the Guangdong microbiology culture center (GDMCC) On July 16, 2016 and has a preservation number of GDMCC NO: 60059. The method utilizes an easily available medium, has stable fermentation conditions and has simple processes and a high yield. The produced grease has high unsaturated fatty acid oil content and especially has high gamma-linolenic acid content. The gamma-linolenic acid can be used for production of foods, medicines and cosmetics, has a wide application range and has a good promotion and application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology. More specifically, it relates to a method for preparing oil rich in γ-linolenic acid by fermenting Penicillium citrinum Asc2-4 with ascidian symbiotic. Background technique [0002] Sea squirt is a kind of marine organism, which belongs to chordate, urochordate, and sea squirt. It grows on pearl shell cages in natural sea areas. Studies have shown that marine invertebrate symbiotic microorganisms average hundreds of species per animal, and those with high density account for 40% of the total weight of the host animal, and most of them belong to the category of microorganisms that are difficult to cultivate. Sea squirts are animals that live in sedentary bodies. The outside of the sea squirt is sheathed by a layer of vesicles similar to plant cellulose, which is unique in the animal kingdom. The unique feeding and filtering system of sea squirts enriches a large number of microorganisms in the body ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/80
CPCC12P7/6427C12P7/6463
Inventor 雷晓凌钟敏聂芳红柴慧子刘唤明
Owner GUANGDONG OCEAN UNIVERSITY
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