Phosphate-solubilizing penicillium PSF and applications thereof

A technology of Penicillium phosphate solubilization and Penicillium phosphate, applied in the field of Penicillium phosphate solubilization PSF strains, can solve the problems of rapid degradation of phosphorus-solubilizing ability, high strain elimination rate, lack of available phosphorus, etc. Phosphorus deficiency, the effect of promoting growth

Active Publication Date: 2020-04-03
BINZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the lack of available phosphorus in the above-mentioned salinized soil. Due to its special physical and chemical properties, the saline-alkali soil may cause traditional non-indigenous phosphorus-solubilizing bacteria to have poor colonization ability, weak competitiveness, and rapid degradation of phosphorus-dissolving ability. To

Method used

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  • Phosphate-solubilizing penicillium PSF and applications thereof
  • Phosphate-solubilizing penicillium PSF and applications thereof
  • Phosphate-solubilizing penicillium PSF and applications thereof

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Experimental program
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Effect test

Embodiment 1

[0031] A kind of Penicillium phospholyticum PSF provided by the invention

[0032] 1. Origin of strains

[0033] The samples come from the salinized soil of the Yellow River Delta;

[0034] Selected from the salinized soil of the Yellow River Delta, weighed 10g of the soil sample and placed it in 90mL of sterile water, and oscillated at a speed of 3000 rpm for 3 minutes to make a soil suspension;

[0035] 2. Isolation of strains

[0036] Dilute the soil suspension by 10-fold gradient, and then pipette 10 -4 、10 -5 、10 -6 Suspend 200 μL of the three gradient soils, spread them on the PVK inorganic phosphorus solid medium respectively, then place the inoculated PVK inorganic phosphorus solid medium upside down in a biochemical incubator, and cultivate for 7 days at 28°C. Fifteen strains of phosphorus-dissolving fungi were screened out, among which the strain number PSF had the strongest phosphorus-dissolving ability, and the phosphorus-dissolving circle formed on PVK solid ...

Embodiment 2

[0049] Solubilization Effect of Penicillium phospholyticum PSF on Various Insoluble Phosphates

[0050] This strain is mainly for four insoluble phosphates namely Ca 5 (PO 4 ) 3 OH, AlPO 4 , Zn 3 (PO 4 ) 2 and FePO 4 Phosphate-dissolving effect;

[0051] Preparation of Penicillium phospholyticum PSF bacterial suspension: according to volume fraction ratio, add 5 parts of pbs buffer solution to 1 part of Penicillium phospholyticum PSF, mix evenly, make Penicillium phospholyticum PSF bacterial suspension, set aside;

[0052] Ca5(PO 4 )3OH、AlPO 4 , Zn 3 (PO 4 ) 2 、FePO 4 The dissolution situation, take the preparation of Ca5(PO4)3OH phosphate detection solution as an example:

[0053] Add 50mL PVK liquid medium, 0.5g Ca 5 (PO 4 ) 3 OH, 500 μL of Penicillium phospholyticum PSF bacterial suspension, cultured on a shaker at a speed of 200 r / min for 5 to 7 days at a temperature of 28 ° C, to obtain Ca 5 (PO 4 ) 3 OH phosphate detection solution;

[0054] Accordin...

Embodiment 3

[0060] Preparation of Fermentation Broth of Penicillium phospholyticum PSF

[0061] Inoculate Penicillium phospholyticum PSF on PDA medium, culture at 28°C for 3 days, inoculate the agar block with a diameter of 7mm containing PSF mycelia by punching method, place it in PVK liquid medium at 28°C, and shake The rotational speed was 180 rpm, and the shaking flask was fermented for 96 hours to obtain the Penicillium phospholyticum PSF fermentation broth, which was set aside.

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Abstract

The invention relates to the technical field of microbial fertilizer, and specifically relates to phosphate-solubilizing penicillium PSF. The strain is named, in classification, as PSF and belongs toPenicillium sp., namely to Penicillium citrinum; and the phosphate-solubilizing penicillium PSF is deposited with the China General Microbiological Culture Collection Center on Oct. 9th, 2019 and assigned accession number CGMCC No. 18558, and the deposited address is No. 3, No. 1 yard, West Beichen Road, Chaoyang District, Beijing 100101. The provided phosphate-solubilizing penicillium PSF has good dissolution effects on various insoluble phosphate such as Ca5(PO4)3OH, AlPO4, Zn3(PO4) and FePO4; and the phosphate-solubilizing penicillium PSF has high salt tolerance and can well grow and colonize in salinized soil so as to make insoluble phosphorus converted into dissolved phosphate that can be absorbed and utilized by plants, so that the growth of the plants can be promoted, the using amount of chemical fertilizer can be reduced, and therefore, the phosphate-solubilizing penicillium PSF can have good application and development prospects.

Description

technical field [0001] The invention relates to the technical field of microbial fertilizers, in particular to a strain of Penicillium sp. and its application in improving the phosphorus supply level of salinized soil. Background technique [0002] Phosphorus is one of the three major elements in plant nutrition, and it is also an important component of organic compounds in plants, and it plays a role that cannot be ignored in its growth and development. 74% of the cultivated land in my country is deficient in phosphorus, and 95% of the phosphorus in the soil is ineffective form. In order to obtain high yield, a large amount of soluble phosphorus fertilizer is repeatedly applied to the soil every year. Only 5% to 10%, mostly with Ca in the soil 2+ , Fe 2+ , Fe 3+ 、A1 3+ Combines to form insoluble phosphates. This not only causes a waste of phosphorus resources, but also poses a certain threat to the safety of the water environment. Therefore, how to improve the availabi...

Claims

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Application Information

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IPC IPC(8): C12N1/14C09K17/14C12R1/80C09K101/00
CPCC09K17/14C09K2101/00C12R2001/80C12N1/145Y02A40/20
Inventor 范延辉王君吴涛李学平张玉苗尚帅刘龙祥
Owner BINZHOU UNIV
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