The invention relates to anticancer liquiritigenin oral preparation and injection preparation, and a preparation method thereof, belonging to a medical technology field. The oral preparation comprises a particulate agent, tablets, capsules, pills and softgel, and the injection preparation comprises small hydro-acupuncture, freeze-dried powder injection, glucose injection and sodium chloride injection. The liquiritigenin oral preparation contains 0.1-100g liquiritigenin per 1000 preparation unit, and the injection preparation contains 0.1-100g liquiritigenin per 1000mL. Pharmaceutic adjuvants contain a diluent, a wetting agent, a disintegrating agent, a flow aid, a cosolvent, an excipient and an osmotic pressure regulator. The liquiritigenin preparation provided by the invention is safe and reliable, and has functions for preventing and treating cancers. And the anticancer medicament is used for preventing and treating lung cancer, liver cancer, stomach cancer, colorectal carcinoma, leukemia, etc., and is a new and effective anticancer medicine.