Antitumor application of penicillium enol A2 from penicillium citrinum

A technology of Penicillium citrinum and IBPT-5, which is applied in the direction of anti-tumor drugs, methods based on microorganisms, and medical preparations containing active ingredients, etc. It can solve problems such as drugs that have not yet been seen, and achieve significant anti-tumor activity.

Inactive Publication Date: 2015-04-22
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have found that the alkaloid compounds shown have anti-tumor activity, but there is no known effect of this compound on human colon cancer cells SW620, human liver cancer cells Huh7, human colon cancer cells SW48...

Method used

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  • Antitumor application of penicillium enol A2 from penicillium citrinum
  • Antitumor application of penicillium enol A2 from penicillium citrinum

Examples

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Effect test

Embodiment 1

[0020] Fermentative production and separation and purification of the compound of embodiment 1

[0021] 1 Fermentation production

[0022] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take Penicillium citrinum ( Penicillium citrinum ) IBPT-5 (preserved in China Center for Type Culture Collection on December 25, 2013, address: Wuhan University, Wuhan, deposit number: CCTCC NO: M 2013713) appropriate amount, inoculated on PDA solid slant medium at 28 Cultivate in an incubator for 4 days.

[0023] Get the Penicillium citrinum ( Penicillium citrinum ) appropriate amount of IBPT-5, inoculated into 400mL culture medium [Culture medium composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5, MgSO 4 0.3, NaCl 30.0 constant volume] in a 1000mL Erlenmeyer flask, after 30 days of static culture at 28°C, the mycelia and fermentation broth were obtain...

Embodiment 2

[0032] Example 2 Test of anti-tumor activity in vitro

[0033] 1 Experimental samples and experimental methods

[0034] Preparation of the tested sample solution The test sample is the pure product of the compound separated and refined in the above-mentioned implementation 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0035] Cell lines and cell subculture Tumor cell lines were used, and tumor cells were subcultured in DMEM medium containing 10% FBS at 37°C in an incubator filled with 5% carbon dioxide.

[0036] Cell Proliferation Inhibitory Activity Test Method

[0037] Tetrazolium salt (MTT) method Take tumor cells in the logarithmic growth phase and adjust the cell density to 2×10 per ml 5 Cells were inoculated into 96-well cell culture plates at 200 microliters per well, and filled with 5% CO at 37°C 2 for 4 hours in an incubator. Add 2 microliters of sample solution...

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Abstract

The invention relates to an antitumor application of penicillium enol A2 from penicillium citrinum. Penicillium citrinum IBPT-5 is preserved at the China Center for Type Culture Collection on December 25, 2013; the address is Wuhan University; and the preservation number is CCTCC NO:M 2013713. An experiment proves that the compound has relatively good antitumor activity on a plurality of tumor cells, and can be used for preparing cell proliferation inhibition drugs or antitumor drugs to carry out antitumor research; and the tumor cells comprise human colon cancer cells SW620, human hepatoma carcinoma cells Huh7, human colon cancer cells SW480, human esophageal squamous cancer cells KYSE450, human esophageal carcinoma cells EC9706 and human hepatoma cells PLC.

Description

technical field [0001] The invention relates to a new antitumor application of an alkaloid compound penicillinol A2 derived from Penicillium citrinum. Background technique [0002] Alkaloids are a type of nitrogen-containing organic compounds produced by biological secondary metabolism. There are many types of alkaloids in nature, most of which come from plants, so they are also called plant alkaloids. Alkaloids have important physiological effects on humans and animals, including anti-asthma and antitussive, hypoglycemic, hypolipidemic, antibacterial, antitumor, analgesic, etc., among which antibacterial and antitumor activities are the most prominent. Natural structural alkaloids are an important source for discovering lead compounds in the research of innovative drugs, and there are nearly a hundred kinds of alkaloid drugs currently used in clinical practice. Studies have found that some marine fungi can produce alkaloids with novel structures and good activities in the ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C07D207/44C12P17/10A61K31/4015A61P35/00
CPCC07D207/44C12P17/10C12N1/145C12R2001/80
Inventor 张其清陈立周彤毕延雪
Owner FUZHOU UNIV
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