Cotton leafstalk tissue cultivation and high-differentiation cotton material selective breeding method

A tissue culture, high differentiation rate technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve the problems of genetic research and high differentiation rate material selection that cannot be used for differentiation rate, and shorten the transformation period. The effect of improving transformation efficiency and simple screening method

Inactive Publication Date: 2006-11-22
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

Under the existing culture system, the cultured individual will no longer exist when taking explants for tissue culture, so it cannot be used for genetic research on differentiation rate and material selection with high differentiation rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1, the screening of high differentiation rate strains in China Cotton Institute 24

[0024] Callus induction medium: Added indole acetic acid 0.1mg / L, kinetin 0.1mg / L, 2,4-D 0.1mg / L, glucose 30g / L, Gelrite 2mg / L on the basis of MSB basic medium L, pH 5.8.

[0025] Differentiation medium: Indole acetic acid 0.005mg / L, kinetin 0.005mg / L, glucose 30g / L, Gelrite 2mg / L, pH 6.8 were added on the basis of MSB basic medium.

[0026] Embryoid body germination and seedling growth medium: on the basis of MSB basic medium, kinetin 0.01mg / L, 6-BA 0.01mg / L, sucrose 30g / L, Gelrite 1.5-2.5mg / L, pH 6.8 were added.

[0027] The hormones and other components in the above-mentioned culture medium were all added to the culture medium before sterilization. Before the culture medium was sterilized, the pH value was adjusted with 1M KOH, and then at 121°C, 1.1kg / cm 2 High temperature and high pressure sterilization for 14min.

[0028] Taking Zhongmian Institute 24 as an example, the...

Embodiment 2

[0051] The breeding of high differentiation rate material in embodiment 2, Zhong 394

[0052] Use the same method as in Example 1 to screen 394 individual plants out of 150 strains, and then continue to use the same method to screen out strains with higher differentiation rates for the selected individual plants with high differentiation rates. More than 80% of the lines (Z1-Z7). In 2003, he went to Jiadai in Hainan. In 2004, he multiplied in the experimental field of China Cotton Research Institute in Anyang, Henan, and used the petiole as the explant to screen with the same method. As a result, the lines Z3 and Z7, Z1-Z7 with a differentiation rate of more than 90% were obtained. The differentiation rates of the strains are shown in Table 5.

[0053] material code

[0054] In 2004, the high differentiation rate Z7 was used as the female parent, and the hard-to-differentiate Zhongmiansuo 12 was used as the male parent, and F 1 Dai Dong sent Hainan Jiadai to get F...

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PUM

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Abstract

The present invention discloses cotton tissue culturing process and its application in screening out high differentiation rate cotton line. The cotton tissue culturing process includes the following steps: 1. adopting sterilized leaf stem as explant and inducing callus in callus inducing culture medium; 2. completing the differentiating culture of the callus in differentiating culture medium; and 3. further culturing of the differentiated embryonic callus in embryoid germinating and seedling forming culture medium to obtain regenerated plant. The process has high differentiation rate, and may be applied in screening out high differentiation rate cotton line. In addition, the screened high differentiation rate cotton can raise the converting efficiency of agrobacterium mediating process and shorten the converting period greatly. The present invention is significant in tissue culture, selective breeding and variety improvement of cotton.

Description

technical field [0001] The invention relates to a cotton tissue culture method and its application, in particular to a cotton tissue culture method and its application in screening high differentiation rate cotton strains. Background technique [0002] Genotype plays a decisive role in cotton somatic embryogenesis and plant regeneration ability. First of all, the somatic embryogenesis and plant regeneration ability of different cotton species in the cotton genus are different. By comparing multiple cotton species, the researchers found that only Upland cotton, Raymond cotton, and Hawaiian cotton could form somatic embryos. Dong Hezhong and Chen Zhixian compared the somatic embryogenesis ability of four cotton cultivars and found that upland cotton is easier to induce somatic embryos, followed by Asian cotton, sea island cotton and African cotton are worse (Dong Hezhong. Hypocotyls of different genotypes of cotton Research on Embryogenesis in Vitro Culture. Journal of Laiya...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/02A01H1/04A01H3/04C12N5/04
Inventor 张朝军李付广喻树讯范术丽王玉芬武芝侠李凤莲刘传亮
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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