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Method for improving brassica napus type temporary maintainer line of nuclear recessive epistatic interaction sterility in rapes

A technique of recessive epistasis interaction and nuclear sterility, applied in plant genetic improvement, botany equipment and methods, applications, etc., to achieve short-term and high-efficiency effects

Inactive Publication Date: 2015-03-25
贵州省油菜研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problems to be solved by the present invention are: there are many kinds of genotypes of recessive epistatic interaction male sterile materials, the genetic law is very complicated, the uniformity of the prepared hybrids is poor, the breeding cycle is long and difficult, especially in temporary protection The improvement of the rapeseed line has always troubled the vast number of rapeseed researchers. The present invention provides a method for improving the temporary insurance line of the recessive epistatic genic male sterile rapeseed. The method has a short breeding cycle, simple operation, and small workload. The fertility is stable, the genetic background is relatively consistent with the homozygous two-type line, and the combining ability with the restorer line is high. It has great application value in the breeding of new rapeseed varieties, so as to overcome the shortcomings of the existing technology

Method used

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  • Method for improving brassica napus type temporary maintainer line of nuclear recessive epistatic interaction sterility in rapes
  • Method for improving brassica napus type temporary maintainer line of nuclear recessive epistatic interaction sterility in rapes

Examples

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Effect test

Embodiment 1

[0021] The excellent yellow seed homozygous two-type line W12031 (genotype: wxya 3 ms 3 ms 4 ms 4 , BnRfRfms 3 ms 3 ms 4 ms 4 ) improved clinical insurance line H12091 (genotype: Bnrfrfms 3 ms 3 ms 4 ms 4 ).

[0022] (1) Select a B strain with excellent performance from the homozygous two-type line W12031 population of excellent recessive epistatic interaction genic male sterile rapeseed ( wxya 3 ms 3 ms 4 ms 4 ) bagging selfing to get F 1 generation seeds.

[0023] (2) F obtained in step (1) 1 Substitute seeds are planted into a plot, totally 80 strains, wherein 19 strains of sterile strains are all eliminated, 61 strains of fertile strains are selected 10 excellent individual strains bagging selfing, harvest F 2 generation seeds.

[0024] (3) F obtained in step (2) 2 The seeds of different individual plants of different generations were planted into different rows, and the situation of fertility separation was investigated. Se...

Embodiment 2

[0034] W13071 (genotype: wxya 3 ms 3 ms 4 ms 4 , BnRfRfms 3 ms 3 ms 4 ms 4 ) improved clinical insurance line H13081 (genotype: Bnrfrfms 3 ms 3 ms 4 ms 4 ).

[0035] (1) Select a B strain with excellent performance from the homozygous two-type line W13071 population of excellent recessive epistatic interaction genic male sterile rapeseed ( wxya 3 ms 3 ms 4 ms 4 ) bagging selfing to get F 1 generation seeds.

[0036] (2) F obtained in step (1) 1 Substitute seeds are planted into a plot, totally 75 strains, wherein 17 strains of sterile strains are all eliminated, 58 strains of fertile strains are selected 10 excellent single plant bagging selfings, harvest F 2 generation seeds.

[0037] (3) F obtained in step (2) 2 The seeds of different individual plants of different generations were planted into different rows, and the situation of fertility separation was investigated. Fertility separation occurred in 6 rows and eliminated, an...

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Abstract

The invention discloses a method for improving a brassica napus type temporary maintainer line of nuclear recessive epistatic interaction sterility in rapes. The method comprises the following steps: selecting a homozygous two-type line strain B to cap and self-cross, selecting an individual plant from a sterile segregation population to hybridize with a to-be-improved temporary maintainer line; then, taking the sterile segregation plant as a recurrent parent to continuously backcross and self-cross, and test cross to breed a new temporary maintainer line after the backcrossing; hybridizing the temporary maintainer line with the homozygous two type line to obtain a complete sterile line, wherein sterile plants account for 100% of the complete sterile line. The bred temporary maintainer line is stable in heredity, very close to the homozygous two type line genetic background, the obtained complete sterile line is easily compounded with a restoring line to prepare strong superiority hybridized combination, and a novel excellent hybrid rape variety is obtained by screening. The invention provides a novel technical method for widely applying a nuclear recessive epistatic interaction sterility pollination control system with a development prospect to the production practice.

Description

technical field [0001] The invention relates to the field of crop genetics and breeding, specifically to the field of rapeseed crop breeding, in particular to a method for improving the temporary insurance line of recessive epistatic genital sterile rapeseed. Background technique [0002] Male sterility is a common natural phenomenon in the plant world. It is mainly divided into nuclear male sterility and cytoplasmic male sterility. Cytoplasmic male sterility is also called cytoplasmic male sterility. The research was earlier. In 1972, Academician Fu Tingdong of Huazhong Agricultural University first discovered and successfully applied the pol-CMS cytoplasmic male sterility in the practice of rape genetics and breeding. Extensive and in-depth research has been carried out, a series of fruitful results have been obtained, and huge social and economic benefits have been created. Among them, nuclear male sterility is divided into recessive nuclear male sterility and dominant n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 伍林涛奉斌韩宏仕杜才富曾章丽李大雄
Owner 贵州省油菜研究所
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