Method for increasing prodn. of hybridized rice
A hybrid rice, a potential technology, applied in the direction of botany equipment and methods, application, plant genetic improvement, etc.
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Embodiment 1
[0029]Example 1: The three-line hybrid rice restorer line Ce 64-7 was used as the recipient and recurrent parent to cross and backcross with wild rice high-yielding gene donor material. Genomic DNA was extracted from individual plants in each backcross generation, analyzed using closely linked molecular markers, tracking wild rice high-yield genes yld1.1 and yld2.1, and screening heterozygous genotype individual plants containing wild rice amplification bands , and then continuously backcross to BC with the recipient parent as the recurrent parent 3 f 1 . Then self-breed for multiple generations, and conduct field selection mainly based on agronomic traits, select excellent single plants and test-cross with three-line CMS lines such as V20A and Jin 23A, and test cross according to a large number of test-cross F 1 The yield performance of the restorer lines was selected again. Results from BC 3 f 7 The strong and superior restorer line Yuanhui 611 (Q611 for short) was bred...
Embodiment 2
[0054] Example 2: The super hybrid rice restorer line 9311 was used as the recipient and the recurrent parent to cross and backcross with the common wild rice in Malaysia, and track the high-yield genes yld1.1 and yld2.1 of the wild rice with the help of molecular marker-assisted selection technology, from BC 4 f 2 Plants carrying wild rice high-yielding genes were initially selected, and their growth period was comparable to that of the recipient parent 9311. The test cross with Pei'ai 64S showed strong heterosis, and the yield per unit area of the test cross combination was more than 20% higher than that of the control (Table 3).
[0055] Table 3 The results of test cross combination of 9311 near-isogenic line and Pei'ai 64S
[0056] Effective theory produces practice
[0057] thousand grains
[0058] Plant Height Ear Length To...
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