Mutant gene OsCOPT7 of rice copper low accumulation mutant lc1 and application of gene

A mutant gene and mutant technology, applied to the mutant gene OsCOPT7 of rice copper low accumulation mutant lc1 and its application field, achieving remarkable results

Active Publication Date: 2020-11-20
CHINA NAT RICE RES INST
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  • Description
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Problems solved by technology

The CTR-like high-affinity copper transporter (COPT) is the main participant in the process of copper uptake by rice roots. OsCOPT Mutations of family member genes to obtain mutants with low accumulation of copper in grains

Method used

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  • Mutant gene OsCOPT7 of rice copper low accumulation mutant lc1 and application of gene
  • Mutant gene OsCOPT7 of rice copper low accumulation mutant lc1 and application of gene

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Embodiment Construction

[0014] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0015] The present invention uses indica rice 9311 (Oryza_Indica) as wild-type seeds (M 0 Generation) and undergo EMS mutagenesis treatment, plant the treated seeds to obtain M 1 Substitute plants. m 1 Generation plants selfed to produce seeds (M 2 generation), planting M on the field 2 After the harvested rice grains were shelled, the copper content in brown rice was measured by inductively coupled plasma spectrometer (ICP), and the plants with low copper accumulation were screened. The obtained rice lines with low copper accumulation were further screened by selfing, and planted in different paddy fields for verification. A mutant homozygous single plant with low rice copper accumulation and stable inheritance was obtained, named as lc1 ( low copper mutant 1 ), and used in hybrid breeding and biotechnology research.

[0016] Mutants with low c...

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Abstract

The invention discloses a mutant gene OsCOPT7 of rice copper low accumulation mutant lc1 and application of the gene. According to the mutant gene OsCOPT7 of rice copper low accumulation mutant lc1, abase mutation occurs in the coding region of a wild OsCOPT7 gene, namely, the 344th base downstream of ATG is mutated from G to A, and the 115th codon in the coding region is changed from GGG to GAG,so that the amino acid at position 115 in the protein sequence is mutated from glycine (Gly / G) to glutamic acid (Glu / E). the coding nucleotide sequence of the mutant gene OsCOPT7 is shown in SEQ ID No. 1, and the amino acid sequence is shown in SEQ ID No. 2. The copper content in rice grains of mutant lc1 is significantly lower than the copper content in rice grains of the wild type. The application of the mutant comprises one or more of transgene, hybridization, backcrossing or asexual reproduction; and the application of the mutant gene OsCOPT7 comprises enabling rice to contain the mutantgene OsCOPT7, or enabling rice to contain the amino acids encoded by the mutant gene OsCOPT7.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, and in particular relates to a mutant with low copper accumulation in rice lc1 mutant gene OsCOPT7 and its application. Background technique [0002] Copper (Cu) is one of the main elements polluted by heavy metals in cultivated soil in my country. Many studies have shown that excessive copper accumulation inhibits plant growth and affects related physiological processes. In addition, copper, as a coenzyme factor, participates in a variety of biochemical reactions, exists in the human body in the form of ceruloplasmin and copper enzyme, and is an essential trace element for life and health. Adequate copper intake is crucial to the health of residents. Rice is the main food of Chinese residents, and it is of great significance to cultivate low-copper-accumulated rice varieties suitable for copper-contaminated soils. Because the copper accumulation ability of different rice varieties ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/10A01H6/46
CPCC07K14/415C12N15/8243C12N15/8259
Inventor 陈铭学关美艳牟仁祥曹赵云朱智伟
Owner CHINA NAT RICE RES INST
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