Breeding method and application of high-quality Chinese pumpkin variety without vines and seeds
A high-quality technology of Chinese pumpkin, applied in the fields of application, botany equipment and methods, and plant gene improvement, can solve the problem that there are no special varieties of Chinese pumpkin for seeds, achieve no vines in plants, speed up the breeding process, and save breeding costs Effect
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Embodiment 1
[0034] Example 1: Breeding of Chinese pumpkin material 1292 and Quanyou 1 without vine oblate seeds
[0035] 1. In the winter of 2005, the homozygous material 1092 of the oblate Chinese squash without vines and the homozygous material 1066 of the oblate Chinese squash with long vines were crossed at the Sanya base in Hainan in the winter of 2005; 2.5cm long and 1.3cm wide;
[0036] 2. F 1 It was planted in Hefei, Anhui in the spring of 2006. It showed no vines, oblate fruit, seed size between 1092 and 1066, and 350 seeds per fruit. Using 1066 as the recurrent parent to backcross once to obtain BC 1 ;
[0037] 3. BC 1 In the winter of 2006, the performance segregation was planted in Sanya Base, Hainan, and then the recurrent parent 1066 was backcrossed with all BC1 plants to obtain BC 2 , select a single fruit without vines, with a seed board length of 2.2-3cm and a board width of 1.1-1.5cm;
[0038] 4. BC 2 Planted in Hefei, Anhui in the spring of 2007 and still showed s...
Embodiment 2
[0042] Example 2: Breeding of Chinese pumpkin material 1295 and Quanyou 2 without vines
[0043] 1. In the spring of 2010, the homozygous material 1092 of Chinese squash without vines was crossed with the homozygous material 1095 of Chinese squash with long vines and long tubes at the base in Hefei, Anhui in the spring of 2010; The number of seeds of a single fruit is 520;
[0044] 2. F 1 Planted in Sanya Base, Hainan in the winter of 2010, the plants showed no vines, and the fruit was flat pear-shaped. Using 1095 as the recurrent parent to backcross once to obtain BC 1 ;
[0045] 3. BC 1 In the spring of 2011, it was planted and separated at the base in Hefei, Anhui Province, and it was beneficial to BC 1 All are self-crossed, and then the reincarnated parent 1095 is used with all BC 1 Planted single plant backcross to obtain BC 2 , select BC with 450 seeds per fruit 2 Single plant is self-crossed to obtain BC 2 f 2 ;
[0046] 4. Select BC with no vines, long tube s...
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