Transformation method of zinnia male sterile dual-use system

Abstract

The invention belongs to the technical field of flower breeding, and particularly relates to a transformation method of a zinnia male sterile dual-use system. The invention takes a sterile plant of a zinnia male sterile dual-use system AH209AB as a parent plant, takes a zinnia inbred line as a father plant, and after cross breeding, inbreeding, continuous backcrossing, group pair hybridizing and restoring and maintaining test, an orangered single recessive zinnia male sterile dual-use system J16AB is finally transformed and obtained. The invention can transform a male sterility gene into an inbreeding line with excellent properties, obtains the zinnia male sterile dual-use system with consistent gardening properties, thorough abortion and stable fertility inheritance, enriches the breeding materials, and lays the foundation for the zinnia crossbreeding. During the transformation process, through the phenotype characteristics of floral organs, the invention can accurately analyze and judge the fertile plants and the sterile plants of the zinnia, and provides phenotype marks for zinnia fertility identification.

Description

technical field [0001] The invention relates to a flower breeding method, in particular to a method for transferring male sterile dual-purpose lines of zinnia, belonging to the technical field of flower plant breeding. Background technique [0002] Plant male sterility refers to the phenomenon that the male organs of flowering plants are degenerated, stunted or pollen is aborted and cannot perform normal reproductive functions, but their female organs develop normally and can receive normal pollen for fertilization and fruiting. It widely exists in flowering plants Among them, genetic male sterility has been observed in 617 varieties or interspecific hybrids of 320 species in 162 genera in 43 families. The sources of male sterility are mainly conventional breeding and genetic engineering creation, and conventional breeding to obtain male sterile lines mainly includes the use of natural variation, self-breeding and inter-species hybridization, introduction and transfer, dista...

AI Technical Summary

Problems solved by technology

However, most of the excellent varieties of zinnia have Melaleuca petals, and the ligulate flower petals cover one layer, covering the stigma, which is difficult to pollinate; the varieties with low double petals have well-developed tubular flowers but difficult emasculation. The method of utilizing heterosis must apply male sterility technique

[0004] Although male sterility technology has been widely used in Zinnia seed production abroad, due to the protection of intellectual property rights, there are very few related academic reports, and domestic breeding research is still in its infancy. , Xu Haixia et al. (2007) and Ye Yaomei et al. (2008) have done some research on the male sterility of zinnia

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