Seed Production Method of Jingnongke 728 Three-line Matching Hybrid

A technology of hybrid seed production and inbred lines, applied in the biological field, can solve the problems of long transfer cycle

Active Publication Date: 2016-08-24
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, only using the traditional backcrossing transfer method usually requires backcrossing for 5-6 generations, and the transfer cycle is longer, which delays the application of sterile seed production technology in corn hybrids

Method used

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  • Seed Production Method of Jingnongke 728 Three-line Matching Hybrid
  • Seed Production Method of Jingnongke 728 Three-line Matching Hybrid
  • Seed Production Method of Jingnongke 728 Three-line Matching Hybrid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1, the breeding of male sterile line S Jing 724

[0073] 1. Determination of the type of male sterile line donor MD32 cytoplasmic male sterility

[0074] 1. Maize male sterile line MD32

[0075] Breeding process of maize male sterile line MD32: use the constructed X line population (based on X1132) to carry out "high and strict" line selection, and select excellent S5 high-generation lines and introduced hybrids to construct a new basic line selection population. Sterile plants were found in the selfed progeny, and the anthers of the tassels were not exposed, and sister plants with similar phenotypes in the same panicle row were selected for pair pollination. The hybrid offspring all showed complete sterility, and the plant phenotype tended to be basically the same. Then the material was named maize male sterile line MD32.

[0076] The corn sterile line MD32 is completely sterile: the anthers are not exposed;

[0077] The sterility of maize sterile line M...

Embodiment 2

[0121] Embodiment 2, the transfer of female parent Beijing MC01 male sterile line S Beijing MC01

[0122] Since Jing MC01 (recorded in the following literature: "Seed World" 2014 Issue 4 summer maize Jing Nong Ke 728 basic characteristics and high-yield cultivation techniques, the public can obtain from Beijing Academy of Agriculture and Forestry Sciences) and Jing 724 are new foreign germplasm X The second ring line of the population was selected, so the sterile line S Jing 724 was selected as the donor material for the transfer of the Beijing MC01 sterile line.

[0123] Using S Jing 724 as the female parent and Jing MC01 as the male parent to cross F 1 Generation, F 1 The generation field showed complete sterility;

[0124] then F 1 Make the female parent, and continue to backcross with Beijing MC01 to get BC 1 generation groups.

[0125] From 1200 plants planted BC 1 360 individual plant markers with complete male sterile male flowers and plant traits (plant height, e...

Embodiment 3

[0133] Embodiment 3, Jing 2416 to the restorative identification of male sterile line S Jing MC01

[0134] With the male sterile line S Beijing MC01 created by Example 2 as the female parent, Beijing 2416 (Corn Research Center of Beijing Academy of Agriculture and Forestry Sciences, the variety rights authorization announcement number is CNA004319G) as the male parent, the hybrid combination F 1 Generation, Harvest F 1 generation of seeds. The next year, plant F in Beijing, Hainan, Jilin and other fields 1 Generation, get F 1 Substitute plants. Observation identification F 1 Fertility restoration of tassels in generation plants showed that fertility returned to normal.

[0135] It shows that Jing 2416 is a strong restorer line of the male sterile line S Jing MC01 and has full recovery ability.

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Abstract

The present invention discloses a Jingnongke 728 three-line supporting hybrid variety seed production method, and provides a seed production method through hybridization, wherein the method comprises: adopting a corn sterile line S jing MC01 as a sterile line, adopting a corn inbred line S jing MC01 as a maintainance line, adopting a corn inbred line jing 2416 as a restoring line, and carrying out three-line supporting variety production to obtain the hybrid variety Jingnongke 728. The experiment results of the present invention prove that the yield, the resistance and other agronomic traits of the seeds of the sterilized hybrid variety Jingnongke 728 prepared by utilizing the bred sterile line S jing MC01, the maintainance line jing MC01 and the restoring line jing 2416 to carry out Jingnongke 728 three-line supporting hybrid variety production are substantially the same as the seeds of the Jingnongke 728 produced through the conventional variety production method.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a seed production method for Jingnongke 728 three-line matching hybrid. Background technique [0002] Breeding and promotion of strong dominant hybrids is an important way to increase maize yield. The use of conventional seed production methods to prepare hybrids requires manual detasseling of the female parent, which not only consumes a lot of labor, increases the cost of seed production, but also has the potential risk of affecting seed quality due to untimely and incomplete detasseling. The use of male sterile lines for seed production is an effective method to ensure seed purity and increase maize yield. [0003] As early as the 1950s and 1960s, research on maize sterile seed production began at home and abroad. Cytoplasmic male sterility is the main type used in maize breeding because it is easy to realize the matching of sterile lines, maintainer lines and restorer lines. Cy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02C12Q1/68
Inventor 赵久然宋伟邢锦丰王元东段民孝刘春阁冯培煜张如养王凤格毛振武李瑞媛王乃顺王文广张莎莎
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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