Application of rice gene osdes1

Abstract

The invention discloses the application of the rice gene OsDES1, and the invention reveals for the first time that the rice gene OsDES1 has a biological function of regulating plant fertility. Based on gene knockout experiments and overexpression experiments, the function of the gene was verified in rice. By constructing a CRISPR / Cas9 gene knockout vector to transform rice Nipponbare, the female gametes of the transgenic plants had abnormal development and reduced fertility; the pCUBi1390‑OSDES1 overexpression vector was used After transformation of the mutant des1, the fertility of the female gametes of the transgenic plants was restored, that is, the loss-of-function mutation of the OSDES1 gene led to partial abortion of the females, while the overexpression of the gene restored the fertility of the female gametes. The rice OSDES1 gene function of the invention is not completely aborted, and some seeds can be harvested, which can form a favorable supplement with other female sterile genes, and provide new gene resources for the breeding of intelligent restorer lines of rice.

Description

Application of rice gene OsDES1 technical field The present invention relates to the field of genetic engineering and plant genetics and breeding, specifically, relate to the application of rice gene OsDES1. use. Background technique

[0002] Rice, as the most important food crop, provides nearly 50% of human food and nutrition sources. half of our country The population above eats rice as the main food. The promotion of hybrid rice has made a huge contribution to the world's food security, In the 1970s, the landmark discovery of the male sterility gene in wild abortive rice promoted the promotion of three-line hybrid rice breeding. A large number of hybrid combinations with strong heterosis. The utilization of heterosis is divided into nucleocytoplasmic interaction pollen sterility represented by The "three-line method" hybrid rice and the "two-line method" hybrid rice represented by PTGMS. However, the "three-line law" is subject to strict Restricted by the r...

Examples

Embodiment 1

And the length became shorter, and other flower organs were not abnormal in morphology (Figure 2c and d). Pollen potassium iodide staining analysis, wild type can be

The fertile pollen grains were 98.0±1.2%, and the fertile pollen grains of the mutants were 64.0±7.1% (Fig. 2e, f and g), all reaching the fertile standard

allow.

Table 1 positive and negative cross seed setting rate statistics

[0065]

[0066]

Application of rice gene OsDES1 technical field The present invention relates to the field of genetic engineering and plant genetics and breeding, specifically, relate to the application of rice gene OsDES1. use. Background technique

[0002] Rice, as the most important food crop, provides nearly 50% of human food and nutrition sources. half of our country The population above eats rice as the main food. The promotion of hybrid rice has made a huge contribution to the world's food security, In the 1970s, the landmark discovery of the male sterility gene in wild abortive rice promoted the promotion of three-line hybrid rice breeding. A large number of hybrid combinations with strong heterosis. The utilization of heterosis is divided into nucleocytoplasmic interaction pollen sterility represented by The "three-line method" hybrid rice and the "two-line method" hybrid rice represented by PTGMS. However, the "three-line law" is subject to strict Restricted by the r...

Examples

Embodiment 1

And the length became shorter, and other flower organs were not abnormal in morphology (Figure 2c and d). Pollen potassium iodide staining analysis, wild type can be

The fertile pollen grains were 98.0±1.2%, and the fertile pollen grains of the mutants were 64.0±7.1% (Fig. 2e, f and g), all reaching the fertile standard

allow.

Table 1 positive and negative cross seed setting rate statistics

[0065]

[0066]

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