Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Zinc finger protein DPZF gene knock-out mice model and its usage

A technology of zinc finger protein and gene knockout, applied in the field of transgenic, can solve the problems of limited understanding of zinc finger protein and lack of means

Inactive Publication Date: 2008-01-02
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the understanding of the zinc finger protein DPZF is extremely limited, and the necessary means are lacking

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Zinc finger protein DPZF gene knock-out mice model and its usage
  • Zinc finger protein DPZF gene knock-out mice model and its usage
  • Zinc finger protein DPZF gene knock-out mice model and its usage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Establishment of Zinc Finger Protein Gene Knockout Model

[0066] 1. By constructing a gene targeting vector, see Figure 1;

[0067] 2. Transfect embryonic stem cells (ES) with the above-mentioned vectors, and screen homologously recombined ES clones;

[0068] 3. After injecting the homologously recombined ES cells obtained in step (3) into blastocysts, implant them into the uterus of pseudopregnant mice to obtain chimeric mice;

[0069] 5. The chimeric mice were further mated to obtain zinc finger protein DPZF knockout mice, whose genotype was identified by Southern blot hybridization, as shown in Figure 2, and the deletion of DPZF protein was confirmed by Western blot and immunohistochemical analysis.

Embodiment 2

[0071] Phenotypic Analysis of Zinc Finger Protein Knockout Mice 1

[0072] The DPZF gene-deficient mice obtained in Example 1 had no obvious abnormalities at birth, but showed obvious growth and development disorders one week after birth, and their body weight was about 50% of that of normal mice, as shown in FIG. 3 .

[0073] The results showed that most of the mice died 3 weeks after birth, and the rest survived for no more than 5 months.

Embodiment 3

[0075] Phenotypic Analysis of Zinc Finger Protein Knockout Mice 2

[0076] The DPZF gene-deficient mice obtained in Example 1 have complete hearing loss, as shown in FIG. 4 .

[0077] The results of immunohistochemical analysis showed that DPZF was specifically expressed in the inner ear cells of normal mice (see Figure 5), suggesting that DPZF is necessary for hearing development in mice.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a DPZF gene removing non-human mammal mould and making method, which also provides the application to accelerate growth and development, modify auditory acuity and blood sugar metabolism to treat hypotrophy, dysacousis, obesity and hyperglycemia based on upstream and downstream biological signal transduction path as drug intervention target.

Description

technical field [0001] The invention relates to the field of transgenic technology, and more specifically relates to the establishment and application of a zinc finger protein DPZF gene knockout animal model. Background technique [0002] Zinc finger proteins are a class of DNA-binding proteins widely found in animals, plants and humans. Due to its selective combination with various DNA and RNA sequences, it plays a very important role in the regulation of gene expression, cell differentiation, gamete formation and embryonic development. Since 1985, scientists have conducted a lot of research in this area. It is found that most of the zinc finger proteins studied are gene regulatory factors. They can not only bind to DNA, RNA and DNA-RNA hybrids, but also bind to Other zinc finger proteins bind to control gene transcription, translation and protein synthesis in organisms. [0003] So far, 10 zinc finger structures have been discovered, and the zinc finger protein DPZF is a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12N5/10A01K67/027C07K14/435A61K38/17A61P3/08A61P27/16
Inventor 章卫平
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com