Seed production method of nk718 three-line mating hybrid

A technology of hybrid seed production and inbred lines, applied in the biological field, can solve problems such as long transfer cycle, achieve stable sterility, high combining ability, and save tedious work

Active Publication Date: 2016-08-24
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, only using the traditional backcrossing transfer method usually requires backcrossing for 5-6 generations, and the transfer cycle is longer, which delays the application of sterile seed production technology in corn hybrids

Method used

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  • Seed production method of nk718 three-line mating hybrid
  • Seed production method of nk718 three-line mating hybrid
  • Seed production method of nk718 three-line mating hybrid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1, the breeding of male sterile line S Jing 724

[0057] 1. Determination of the type of male sterile line donor MD32 cytoplasmic male sterility

[0058] 1. Maize male sterile line MD32

[0059] Breeding process of maize male sterile line MD32: use the constructed X line population (based on X1132) to carry out "high and strict" line selection, and select excellent S5 high-generation lines and introduced hybrids to construct a new basic line selection population. Sterile plants were found in the selfed progeny, and the anthers of the tassels were not exposed, and sister plants with similar phenotypes in the same panicle row were selected for pair pollination. The hybrid offspring all showed complete sterility, and the plant phenotype tended to be basically the same. Then the material was named maize male sterile line MD32.

[0060] The corn sterile line MD32 is completely sterile: the anthers are not exposed;

[0061] The sterility of maize sterile line M...

Embodiment 2

[0105] Embodiment 2, the transfer of female parent Jing 464 male sterile line S Jing 464

[0106] Since Jing 464 and Jing 724 are closely related, the CMS line S Jing 724 was selected as the donor material for the transfer of Jing 464 CMS.

[0107] The male sterile line S Jing 724 was used as the female parent, and Jing 464 (Corn Research Center of Beijing Academy of Agriculture and Forestry Sciences, the variety right application announcement number is CNA007810E) was used as the male parent. 1 Generation, F 1 The generation field showed complete sterility;

[0108] then F 1 Make the female parent, and continue to backcross with Jing 464 to get BC 1 generation groups.

[0109] From 200 plants planted BC 1 In the generation population, 60 individual plant listing markers with complete male flower sterility and plant traits (plant height, ear height, axis color, number of tassel branches, ear traits and growth period, etc.) close to Jing 464 were selected; BC 1 The DNA of...

Embodiment 3

[0112] Embodiment 3, Jing 2416 is to the restorative identification of male sterile line S Jing 464

[0113] With the male sterile line S Beijing 464 created by Example 2 as the female parent, Beijing 2416 (Corn Research Center of Beijing Academy of Agriculture and Forestry Sciences, the variety rights authorization announcement number is CNA004319G) as the male parent, the hybrid combination F 1 Generation, Harvest F 1 generation of seeds. The next year, plant F in Beijing, Hainan, Jilin and other fields 1 Generation, get F 1 Substitute plants. Observation identification F 1 Fertility restoration of tassels in generation plants showed that fertility returned to normal.

[0114] It shows that Jing 2416 is a strong restorer line of the male sterile line S Jing 464 and has full recovery ability.

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Abstract

The invention discloses a seed production method for an NK718 three-line support hybrid seed, and provides a method of hybrid seed production. The method comprises the following steps: adopting the corn sterile line S Jing 464 as the sterile line; adopting the corn inbred line Jing 464 as the maintainer line; adopting the corn inbred line Jing 2416 as the restoring line; conducting three-line support seed production to obtain the hybrid seed NK718. The experiment of the method shows that the sterile line S Jing 464, the maintainer line Jing 464 and the restoring line Jing 2416 bred are adopted for seed production of theNK718 three-line support hybrid seed, and the yield, resistance and other agronomic characters of the prepared unbred hybrid seed NK718 are basically the same as those of the NK718 seed prepared in the conventional preparation method.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for producing NK718 three-line hybrid hybrids. Background technique [0002] Breeding and promotion of strong dominant hybrids is an important way to increase maize yield. The use of conventional seed production methods to prepare hybrids requires manual detasseling of the female parent, which not only consumes a lot of labor, increases the cost of seed production, but also has the potential risk of affecting seed quality due to untimely and incomplete detasseling. The use of male sterile lines for seed production is an effective method to ensure seed purity and increase maize yield. [0003] As early as the 1950s and 1960s, research on maize sterile seed production began at home and abroad. Cytoplasmic male sterility is the main type used in maize breeding because it is easy to realize the matching of sterile lines, maintainer lines and restorer lines. Cytoplasmic male s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 赵久然宋伟邢锦丰王元东段民孝刘春阁冯培煜张如养王凤格毛振武李瑞媛王乃顺王文广张莎莎
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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