Method for transferring male sterile line of Chinese pink and cultivating propagating seeds and hybrid seeds
A technology of male sterile lines and hybrids, which is applied in the field of plant cultivation, can solve problems such as time-consuming and labor-consuming, and achieve the effect of increasing the number of flowering branches, normal flowering and fruiting traits of plants, and vigorous growth
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Embodiment 1
[0021] For this example see figure 1 , in order to obtain a male sterile line of Dianthus male with stable sterility, normal plant growth and development, normal flowering and fruiting, excellent and stable economic traits, and strong combining ability, a continuous backcrossing method was adopted to optimize the existing male Dianthus male The male sterile line 20480A or 20468A was used as the female parent, and one of the existing high-generation self-compatibility lines 20708B, 20710B, 20714B, 20715B, and 20718B with stable traits and strong combining ability was selected as the male parent for paired crosses; Select the sterile and complete single plant closest to the male parent's economic traits among the hybrid offspring as the female parent, and then backcross with the original male parent; such continuous backcrossing for multiple generations (for example, five generations) until the main economic traits are similar to those of the backcross. Dianthus male sterile lin...
Embodiment 2
[0031] The method for obtaining the male sterile line of Dianthus in this embodiment is the same as that in Example 1. On this basis, this embodiment further utilizes the male sterile line of Dianthus to obtain its hybrid seeds, see figure 2 , at first the male sterile line of Dianthus as the female parent and the self-compatibility line as the male parent of the hybrid are sown in autumn; The plants are respectively transplanted to a breeding field, preferably a net shed, at a row ratio of 4:1; when the flowers are to be bloomed, honey bees are put into them for pollination; For the preparation of hybrid seeds. The hybrid combination prepared in this example has strong growth vigor, many flowering branches, and obvious heterosis. The comparison of its economic traits with the male parent is shown in Table 3.
[0032] table 3
[0033]
[0034] The above-mentioned use of Dianthus male sterile lines to cultivate Dianthus hybrids should pay attention to the following techni...
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