Novel selective breeding method for male sterile line of Sichuan preserved pickle cytoplasm brasica napus

Abstract

The invention discloses a novel selective breeding method for a male sterile line of Sichuan preserved pickle cytoplasm brasica napus. According to the method, a sterile source of the Sichuan preserved pickle is transferred into the brasica napus through distant hybridization and continuous backcrossing between different inter-species in brassica, so as to obtain Sichuan preserved pickle cytoplasm brasica napus CMS '93-12A'. The Sichuan preserved pickle cytoplasm brasica napus CMS '93-12A' is improved by hybridization and continuous backcrossing, so as to obtain a novel, stable, and double low sterile line material 'Jia rapeseed 58A'; the identification of the restoring and maintaining relationship of the Sichuan preserved pickle cytoplasm CMS shows that the restoring and maintaining relationship of the 'Jia rapeseed 58A' is different from the restoring and maintaining relationships of Pol CMS and Shaan 2A CMS, and meanwhile, one part of restorer material is screened out, and is improved to obtain five parts of double haploid restorer materials; the PCR identification with a specific primer shows that sterile sources of the '93-12A', the 'Jia rapeseed 58A' and mustard CMS are the same; an identification result of hybridization mating group yield of the 'Jia rapeseed 58A' and different restorer materials shows that a part of the combinations have a good yield-increasing potential, and the 'Jia rapeseed 58A' has a good combining ability. The method provided by the invention can be applied to the selective breeding of rapeseed varieties and the planting of the rapeseed in production.

Description

Technical field: [0001] The patent of the present invention is a breeding method of a new plant variety. Background technique: [0002] Brassica napus has strong heterosis [1] , the utilization of heterosis in rapeseed is an important way to increase yield and increase economic income, and the use of excellent cytoplasmic male sterile lines (CMS) to produce hybrids with three lines is the main way to utilize heterosis in rapeseed at home and abroad. my country is at the international leading level in the application and related research of Brassica napus CMS. At present, most of the major domestic rapeseed production areas are mainly hybrids, and at the same time, the quality improvement of "double low + heterosis" is closely combined with the utilization of heterosis. Simultaneous improvement of quality and output. At present, there are many cytoplasmic male sterile lines of Brassica napus that have been studied at home and abroad and are used in large-scale breeding pract...

AI Technical Summary

Problems solved by technology

Judging from the planting conditions of the varieties "Huaza No. 3", "Qinyou No. 2", and "Ningza No. 1" that have been widely promoted and applied in production in recent years, there is a certain amount of pollen in Shan 2A CMS and Pol CMS , leading to the problem of varying numbers of sterile plants in hybrids, which affects the purity of hybrids and brings greater risks to production [2] Ogu CMS has not been well applied in a large area in the early stage due to the problem of the restorer line. In recent years, Europe has obtained its stable restorer line through cell engineering and other biotechnologies, and realized the three-line matching [3，4] , but it is difficult to be applied by us due to patent protection. The domestic Ogu restoration line selection has also made significant progress in recent years, but it has not yet been applied in production [5]

[0003] Brassica napus CMS "93-12A" is a cytoplasmic male sterile line of Brassica napus introduced by Zhejiang University as the source of sterile cytoplasm. Through continuous backcrossing, the CMS "93-12A" of Brassica napus was initially successfully bred. Fertile line, which is characterized by complete sterility, sterility rate and degree of sterility are 100% [9] , but the initial backcross male parent of the male sterile line has relatively large defects in yield traits, quality traits, and combining ability. At the same time, the restorer line material is difficult to find, and it is difficult to use it in production. , quality trait improvement and three-line matching research will play a vital role in its breeding utilization

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