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Natural brassinolide analogue application

一种芸苔素内酯、类似物的技术，应用在农用化学领域，能够解决活性成分单一、制备成本上升大、长期施用效果不如天然芸苔素内酯等问题

Active Publication Date: 2013-07-24
CHENGDU NEWSUN CROPSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chemically synthesized brassinolide, as described in Chinese patent applications CN1217337A, CN1217338A, etc., although the product is relatively uniform, but the active ingredient is single, and the long-term application effect is usually not as good as natural brassinolide, which has been widely used in China. The use of 28-epihomobrasinolide is particularly prominent on
[0004] Brassinolides extracted from plants (such as pollen) or other natural sources (such as beeswax), which usually contain a variety of brassinolide structural analogues, are used as a green production material, and their promotion It is easier to use and is highly praised by users at home and abroad, but due to the limitation of the extraction process, it is easy to produce unstable product quality; if it is further purified, the preparation cost will increase greatly, which is not conducive to the implementation of industrialization, thus hindering people from further mass production. Purification, and hinder people to study whether each brassinolide analog has the activity of brassinolide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Extraction and Identification of Natural Brassinolide Analogs

[0046] Take 100 grams of rapeseed pollen, add 300 mL of 95% (V / V) ethanol, ultrasonically crush, filter, and retain the filtrate; add 300 mL of 95% (V / V) ethanol to the filter residue, ultrasonically crush, filter, and retain the filtrate. Combine the filtrates, dry and concentrate at 75°C under a vacuum of 0.085Mpa to a volume of 100mL to obtain pollen alcohol extract.

[0047] Add 200 mL of 50% (V / V) ethanol to the pollen alcohol extract, mix well, then add 500 mL of ethyl acetate for extraction, keep the upper layer (ethyl acetate layer); continue adding 500 mL of ethyl acetate to the lower layer for extraction, keep the upper layer ( ethyl acetate layer). Combine the ethyl acetate layers, add 300mL enzyme solution (that is, the mixed enzyme solution of lipase and glucoamylase, wherein lipase (purchased from Jining Hemei Bioengineering Co., Ltd.) 2500U / L, glucoamylase (purchased from Jining He...

Embodiment 2

[0063] The determination of the enhanced plant development of embodiment 2 natural brassinolide analogs

[0064] We determined the enhancement effect of natural brassinolide analogues on plant development by measuring the rice leaf inclination angle by the rice leaf bending test. The greater the inclination angle of rice leaves, the stronger the effect on development. The specific test process is as follows:

[0065] Get respectively the natural brassinolide analogs BR1, BR2, BR3, BR4, BR5 and BR6 prepared in Example 1, and in addition BR1, BR2, BR3, BR4, BR5 and BR6 are mixed into a mixed brassinolide analog (abbreviated as It is "BR mixed"; wherein the weight ratio of BR1: BR2: BR3: BR4: BR5 and BR6 is 0.4: 0.4: 0.4: 0.4: 0.4: 98), respectively dissolved in 95% (V / V) ethanol, and then used A large amount of pure water was gradually diluted to 1mg / L, 0.1mg / L, 0.01mg / L, 0.001mg / L and 0.0001mg / L; at the same time, pure water was used as a control.

[0066]After the rice seed...

Embodiment 3

[0070] Example 3 Determination of natural brassinolide analogs endowing plants with anti-low temperature stress activity

[0071] Through low temperature stress experiments, we measured the plant height, leaf number, leaf area, tiller number, root number and other parameters of rice seedlings after low temperature stress to fully reflect the effect of natural brassinolide analogues on plant low temperature resistance.

[0072] Take the natural brassinolide analogs BR1, BR2, BR3, BR4, BR5 and BR6 prepared in Example 1, respectively, and dissolve them in 95% (V / V) ethanol, and then use a large amount of pure water to gradiently dilute to 1 mg / L, 0.1mg / L, 0.01mg / L, 0.001mg / L and 0.0001mg / L and pure water was used as a control.

[0073] After the rice seeds were sterilized with 5% sodium hypochlorite solution, rinsed with distilled water until alkaline, placed in a petri dish, and placed in a thermostat at 30°C ± 2°C for 9 days in dark hydroponics. Then, take out the 9-day-old e...

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Abstract

The invention provides an extraction method of a natural brassinolide analogue extracted from plants and an application, and comprises an application of the natural brassinolide analogue for plant growth promotion, low temperature resistance and high salinity resistance. In addition, the invention also provides an agricultural combination comprising the brassinolide analogue.

Description

technical field [0001] The invention belongs to the technical field of agrochemicals. Specifically, the invention relates to brassinolide analogues extracted from plants and applications thereof, including applications in promoting plant growth, low temperature resistance and high salt resistance. Background technique [0002] Brassinolide is internationally recognized as the most active, broad-spectrum, and non-toxic plant growth regulator. Its physiological benefits are higher than the other five types of plant hormones that have been discovered, and it is known as the sixth largest type of plant hormones in the world. . Brassinolides are widely found in angiosperms, gymnosperms and some lower plants. From the perspective of distribution in plants, they exist in roots, stems, leaves, pollen, pistils, fruits and seeds, such as rapeseed pollen Contains rich brassinolides. [0003] Chemically synthesized brassinolide, as described in Chinese patent applications CN1217337A, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J73/00C07J9/00C07J75/00A01N45/00A01N43/22A01P21/00
CPCA01N43/22A01N45/00C07J9/00C07J75/00C07J73/00A01N65/08C07J73/003A01N35/04A01N65/00
Inventor 何其明
Owner CHENGDU NEWSUN CROPSCI
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