A method for establishing a single-plant cloning system of Erythrodonticus through asexual reproduction

Abstract

The invention discloses a method for establishing a syntrichia caninervis mitt. individual plant cloning system through asexual propagation. The method comprises the following steps: a syntrichia caninervis mitt. pure group is selected, a robustly growing plant is selected and the pure group and the plant are washed and sterilized, then sterilized fine-pointed forceps are used to take the stem tips, stems and stem leaf pieces with strong regeneration capabilities of the treated syntrichia caninervis mitt. individual plant, the stem tips, stems and stem leaf pieces are inoculated to a sterilized BBM solid culture medium and then a culture dish is sealed with a parafilm; the culture dish is put in an illuminating incubator for culture under the conditions that the temperature is 25 DEG C, the illumination intensity is 36mu mol.m<-2>.s<-1> and the light / dark time is 14h / 10h, after culture for 2-3 weeks, new branches will appear on the stem tips, spores and leaf blade bases of the mosses through induction; the protonemata will appear on the stem leaf pieces and new branches will appear when the protonemata are developed to a certain stage; and 2 weeks later, the new branches are takenoff, the stem tips and stems of the new branches are re-transferred to a new solid culture medium and repeated transfer and progressive amplification culture are carried out to form the individual plant cloning system.

Description

field of invention [0001] The invention relates to the biological technology field of single-plant cloning of desert mosses, in particular, the invention relates to the technical field of single-plant cloning of erythrodontic moss. Background technique [0002] In recent years, my country's desertification has become more and more serious, and it is one of the countries with the most serious desertification in the world. The total area of ​​desertified land has reached 3.73 million square kilometers, and the annual expansion rate is 2460 square kilometers. The annual direct economic loss due to desertification is as high as 540 million billion. In view of this serious situation, it is urgent to find an effective way to prevent the desertification of land resources. [0003] Traditional windbreak and sand fixation measures such as afforestation, grass grid sand barriers and clay sand barriers are effective on the surface, but they cannot fundamentally solve the problem of des...

AI Technical Summary

Problems solved by technology

[0006] Bryophytes are small, and the species are not suitable for identification. For example, in the Gurbantunggut Desert, Erythra dentatus is very similar to Phyllophyta genus and Phyllophyta phylloxera in the genus Phyllostachys genus, and it is easy to sample in the field. Confusion, different researchers take different samples in the field, not even the same species, lack of comparability in research results obtained in terms of physiological and molecular regulation mechanisms

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