Rapid propagation method of cassava virus-free seedlings

A technology of virus-free seedlings and cassava, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem of hindering the introduction and exchange of high-quality cassava germplasm resources, affecting the large-scale promotion of virus-free seedlings, and the reproductive efficiency of virus-free seedlings Low-level problems, to achieve a significant detoxification effect, reduce the cost of seedling cultivation, and shorten the effect of the cultivation period

Pending Publication Date: 2015-08-12
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

But the above-mentioned method for obtaining virus-free seedlings requires multiple generations to take growth points for tissue culture, the culture period is long, and the reproductive efficiency of virus-free seedlings is low, which affects the large-scale promotion of virus-free seedlings and greatly hinders high-quality cassava seeds. The introduction and exchange of quality resources

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  • Rapid propagation method of cassava virus-free seedlings

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Comparison scheme
Effect test

Embodiment 1

[0026] A kind of cassava virus-free seedling rapid multiplication method, its steps are:

[0027] 1. Explant collection and pretreatment: Use a blade to cut cassava shoots of 2 to 3 cm as explants, cut off the larger young leaves, rinse with tap water for several minutes, rinse clean, use absorbent paper to dry the surface water or Dry naturally; put the shoot explants in a constant temperature box, and treat them at 50°C for 1 hour to complete the detoxification pretreatment of the explants;

[0028] 2. Surface disinfection of explants and stripping of stem tips: Soak in 75% alcohol solution by volume for 10 seconds on the ultra-clean workbench, rinse once with sterile water, and then disinfect with 0.1% mercury chloride solution by weight for 3 minutes. Rinse with sterile water for 4 times, and absorb the surface moisture with sterilized paper; under aseptic conditions, peel off the young leaves covered by the shoots layer by layer from the outside to the inside of the surfa...

Embodiment 2

[0033] A kind of cassava virus-free seedling rapid multiplication method, its steps are:

[0034] 1. Explant collection and pretreatment: Cut off 2-3cm cassava shoots with a knife, cut off the larger young leaves, wash with tap water and dry the surface moisture; then place in a constant temperature box at 55°C Treat 1.5h to complete explant detoxification pretreatment;

[0035] 2. Surface disinfection of explants and stripping of stem tips: Soak the explants in step 1 with alcohol with a volume concentration of 75% for 15 seconds on an ultra-clean workbench, rinse twice with sterile water, and then disinfect with 0.1% mercuric chloride For 4 minutes, wash with sterile water 5 times, and sterilize the paper to dry the surface moisture; peel off the meristem area length of 0.4-0.5mm with 1-2 leaf primordia shoot tips under a binocular dissecting microscope;

[0036] 3. Shoot tip induction culture: the shoot tip cut off in step 2 is inoculated into the shoot tip induction mediu...

Embodiment 3

[0040] A kind of cassava virus-free seedling rapid multiplication method, its steps are:

[0041] 1. Explant collection and pretreatment: Select cassava plants, cut off the 2-3cm shoots, cut off the larger leaves, rinse with tap water, absorb the surface moisture with absorbent paper or dry naturally; then The buds were placed in an incubator at 60°C for 3 hours to complete the detoxification pretreatment of the explants;

[0042] 2. Surface disinfection of explants and stripping of stem tips: Soak the explants in step 1 with 75% alcohol for 15 seconds on an ultra-clean workbench, rinse them twice with sterile water, and then use 0.1% mercuric chloride Sterilize for 5 minutes, wash with sterile water 5 times, and blot the surface moisture with sterile paper; peel off the shoot tip with 1 to 2 leaf primordia in the meristem region with a length of 0.4 to 0.5 mm under a binocular dissecting microscope;

[0043] 3. Shoot tip induction culture: the shoot tip cut off in step 2 is ...

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Abstract

The invention relates to a rapid propagation method of cassava virus-free seedlings. The method comprises the following steps: step one, collecting explants and carrying out a detoxification pretreatment; step two, disinfecting the surfaces of explants and taking off the stem tips; step three, inducing the stem tips to obtain seedlings; step four, subjecting the seedlings to subculture multiplication culture; step five, carrying out virus detection through a semi-quantitative RT-PCR method; step six, domesticating and transplanting the virus-free tube seedlings. The provided method can effectively remove various viruses and bacteria taking cassava stems as the nutrient source, and obtains virus-free cassava seedlings. The healthy planting, purification and rejuvenation of cassava seedling can be achieved. The occupied space for propagation is small, the propagation speed is quick, and the survival rate of transplanting is high. The provided method can be applied to safe species exchange among different countries and areas, can provide healthy cassava seedlings, reduces the happening and spreading of diseases, and provides technical guarantees for sustainable development of cassava.

Description

technical field [0001] The invention relates to a rapid multiplication method of cassava virus-free seedlings, which belongs to the technical field of plant virus-free culture and rapid propagation. Background technique [0002] Cassava mosaic disease is one of the most important viruses in cassava, which often leads to a loss of more than 50% of cassava yield, and can exceed 80% in severe cases, seriously threatening the development of cassava industry. For low-income farmers in tropical regions who take cassava as their main food, it not only greatly affects the economic benefits of cassava planting, but also seriously threatens the food source of the growers, causing extremely serious consequences. Cassava mosaic disease is caused by many different species of Geminiviridae. Seven viruses have been identified, including African cassava mosaic virus (ACMV), East African cassava mosaic virus (ACMV), and East African cassava mosaic virus (ACMV). mosaic Cameroon virus, East A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 朱文丽陈松笔李开绵
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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