Rapid propagation technique of Dendrobium candidum axillary buds

A technology of dendrobium candidum and axillary buds, which is applied to the rapid propagation technology of dendrobium candidum axillary buds and the field of rapid propagation of plants, can solve problems such as incomplete disinfection, stem tip loss of mother plants, variation, etc., and achieve good social and ecological benefits. Conducive to recovery and increase the effect of reproduction coefficient

Active Publication Date: 2012-03-14
SHANGHAI XCT HEALTH FOOD
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Problems solved by technology

The problem is that the offspring propagated by seeds may mutate; the stem tip will lose the mother plant as an explant, and the stem section will be incompletely steriliz

Method used

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Embodiment Construction

[0021] The technical characteristics of the present invention will be further elaborated below in conjunction with specific embodiments.

[0022] 1) Axillary buds survive and grow

[0023] When the new growth of axillary buds between stem nodes of Dendrobium officinale plants is 0.5-1.0 cm long, carefully cut off the complete axillary buds with a blade, and rinse them with tap water. On the ultra-clean workbench, the material was first soaked in 75% alcohol for 30 seconds, then sterilized with 0.1% mercury liter for 10 minutes, soaked in sterile distilled water for 5 minutes each time, and the excess water was blotted dry with sterile filter paper. Finally the material is inoculated on the medium. Using MS as the basic medium, add 0.3mg / L NAA, 1.0mg / L 6-RA, 3% sucrose and 7g / L agar respectively, adjust the pH value to 5.5-6.0, and sterilize under high pressure for 20-25min . The temperature of the cultivation room is (25±3)°C, with the plant cultivation lamp as the light so...

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Abstract

The invention discloses a rapid propagation technique of Dendrobium candidum axillary buds. According to the technique, Dendrobium candidum axillary buds are used as explants directly to induce fasciculated buds; influences of combinations of different types of hormones with different concentration and different natural extracts on survival and growth of axillary buds, induction and multiplication of fasciculated buds and seedlings are compared, the quantity and quality of seedlings growing from fasciculated buds are researched and explored, the optimal culture medium formula in each stage is screened, and therefore, a fasciculated-bud-way high-efficiency rapid propagation system is established, so that plants obtained through rapid propagation are consistent with parent plants genetically so as to provide basis for restoration of wild population of Dendrobium candidum, and furthermore, wild resources are protected. Compared with vegetative propagation, the rapid propagation technique provided by the invention greatly improves propagation factor. A high-efficiency rapid propagation system of Dendrobium candidum which is an endangered plant provides a technical probability for the large-scale production and is beneficial to the restoration of wild population.

Description

technical field [0001] The invention relates to a rapid propagation technology of plants, in particular to a rapid propagation technology of dendrobium officinale axillary buds, and belongs to the field of biotechnology. Background technique [0002] Dendrobium officinale Kimura et Migo or (Dendrobium candidum Wall ex Lind.), also known as black knot grass, belongs to Orchidaceae. Dendrobium is a perennial epiphytic herb, often epiphytic on tree trunks or rocks in dense forests, and often associated with bryophytes. Its fresh stem or dried stem is used as medicine, and it is the most commonly used raw material of traditional medicinal materials and Chinese patent medicines in my country. "Chinese Pharmacopoeia" stipulates that Dendrobium candidum can be processed into earrings, that is, Tiepi Fengdou, after drying and softening. Its taste is sweet and mild, and its nature is slightly cold. It is used for yin deficiency, dry mouth, polydipsia, retching due to lack of food, ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 朱恩灿魏渊源
Owner SHANGHAI XCT HEALTH FOOD
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