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A method for inducing Chlorophyll algae to efficiently synthesize astaxanthin

An astaxanthin and green algae technology, applied in microorganism-based methods, biochemical equipment and methods, unicellular algae, etc., can solve the problems of different astaxanthin synthesis mechanisms, differences in cell physiological and biochemical characteristics, etc., and achieve food safety. , The method is simple and the effect of reducing production costs

Active Publication Date: 2019-09-13
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that although chlorophyll and Haematococcus pluvialis belong to single-celled green algae, the physiological and biochemical characteristics of the two cells are very different, and the synthesis mechanism of astaxanthin is also completely different

Method used

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  • A method for inducing Chlorophyll algae to efficiently synthesize astaxanthin
  • A method for inducing Chlorophyll algae to efficiently synthesize astaxanthin
  • A method for inducing Chlorophyll algae to efficiently synthesize astaxanthin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The influence of embodiment 1 initial cell density on chlorophyll algae biomass concentration and astaxanthin output

[0028] 1.1 Strain activation and seed solution preparation

[0029] The chlorophyll algae C. zofingiensis preserved in the laboratory were transferred to the slant with modified Bristol medium for cultivation, the cultivation temperature was 26°C, and the light was 10 μmol m -2 the s -1 , and observe the growth of green algae.

[0030] Use an inoculation loop to inoculate the chlorophyll algae moss into a conical flask filled with modified Bristol liquid medium for cultivation, at a temperature of 26 °C and a light of 10 μmol m -2 the s -1 In a constant temperature shaking shaker, cultivate for 3 to 5 days and use it as a seed solution.

[0031] The composition of the improved Bristol medium (pH 6.5) described therein is shown in Table 1 below (in mg / L):

[0032] components Content (mg / L) components Content (mg / L) components C...

Embodiment 2～5

[0040] Embodiment 2-5 Effect of nitrogen source concentration on the accumulation of astaxanthin in the green alga C. zofingiensis induced by white light fruit

Embodiment 2-5

[0041] Embodiment 2-5 operates according to the following steps:

[0042] 2.1. Activation of algal species and preparation of seed solution

[0043] The chlorophyll alga C. zofingiensis species preserved in the laboratory was cultivated for 3 to 5 days according to the cultivation method described in 1.1, and used as the seed solution.

[0044] 2.2 Induction culture of accumulating astaxanthin under white light and different nitrogen source concentrations

[0045] The cultivated chlorophyll algae seed liquid is collected according to the method described in 1.2.

[0046] Dilute and resuspend with improved Bristol medium containing different nitrogen source concentrations, and control the cell density to about 3g / L, wherein Examples 2 to 5 respectively use nitrogen source concentrations of 0, 0.1, 0.2, 0.3g / L The modified Bristol medium, glucose 10g / L, and other components are the same as those in Table 1.

[0047] Divide the inoculated induction medium containing differ...

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Abstract

The invention provides a method for efficient astaxanthin synthesis by induction of chromochloris zofingiensis. The method includes steps: S1, activation culture, namely performing inoculation of chromochloris zofingiensis cells into a culture medium to obtain seed liquid by activation culture, to be more specific, subjecting the chromochloris zofingiensis cells to activation culture to a logarithmic phase, and taking the culture liquid in the logarithmic phase as the seed liquid; S2, induction culture, to be more specific, performing inoculation of the seed liquid obtained at the step S1 into an induction culture medium to carry out photoinduction culture, wherein a light source is white light or blue light, intensity of the light source is 60-330Mumol.m<-2>.s<-1>, a light-dark cycle is 12:12(h)-24:0(h), and the induction culture medium comprises glucose with concentration being 9-30g / L and sodium nitrate with concentration being 0-0.75g / L, and pH value of the induction culture medium is 6.0-7.0. According to the method, efficient astaxanthin synthesis can be realized by induction of chromochloris zofingiensis.

Description

technical field [0001] The invention relates to an algae biofermentation culture technology, in particular to a method for efficiently synthesizing astaxanthin by inducing chlorophyll algae. Background technique [0002] Astaxanthin is known as a "super antioxidant", and its antioxidant capacity is more than 10 times that of other carotenoids such as β-carotene, zeaxanthin, lutein and canthaxanthin. It has the ability to scavenge free radicals, prevent The powerful ability of lipid peroxidation plays an important role in preventing age-related diseases such as arteriosclerosis, Parkinson's syndrome, and retinal macular degeneration; for maintaining the health of the eyes and central nervous system, enhancing the function of the immune system, strengthening the energy metabolism of the body, It has multiple functions such as anti-cancer and anti-infection. [0003] At present, there are three main production methods of astaxanthin, which are extraction from shrimp and crab s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P23/00C12N1/12C12R1/89
Inventor 魏东陈俊辉
Owner SOUTH CHINA UNIV OF TECH
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