A Phaffia strain that efficiently overexpresses endogenous astaxanthin synthase gene

A technology of astaxanthin synthase and Phaffia yeast, applied in the biological field, can solve the problems of high price and increased demand for natural astaxanthin, and achieve the effect of high conversion efficiency
CN104278015BInactive Publication Date: 2018-05-01CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2018-05-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a phaffia rhodozyma strain obtained by efficiently an over-expressing endogenous astaxanthin synthetase gene. The invention provides a phaffia rhodozyma genetic engineering strain capable of producing astaxanthin with high yield, obtained by over-expressing an endogenous astaxanthin synthetase gene. A phaffia rhodozyma genetic engineering strain (MK19-pGBKT7crtS) capable of producing astaxanthin with high yield is obtained by over-expressing the astaxanthin synthetase gene crtS by virtue of free plasmids and the strain is named CSR19. An endogenous ribosome bind site sequence (Rbs. sequence for short) exists in front of the crtS gene expressed by the strain. By virtue of fermentation cultivation and in comparison with a receptor strain MK19, the astaxanthin yield of the engineering strain is increased by 33.5%, and the engineering strain is good in stability, and thus, the phaffia rhodozyma strain has a good application prospect in the feed industry.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a strain of Phaffia yeast overexpressing an endogenous astaxanthin synthase gene with high efficiency and its application. Background technique

[0002] Astaxanthin (chemical name: 3,3'-dihydroxy-4,4'-diketo-β-carotene), the molecular formula is C 40 h 52 o 4 , the molecular weight is 596.86. It is a lutein carotenoid that widely exists in nature. Some algae, bacteria, fungi and plankton can synthesize astaxanthin by themselves, while other higher organisms such as crustaceans, fish and birds pass through the food chain Ingest astaxanthin, and then store it in the body, making the appearance red and increasing the commercial value.

[0003] Astaxanthin is the highest-level product of carotenoid synthesis, so in nature, astaxanthin has the strongest antioxidant activity, can effectively remove oxygen free radicals in cells, and can inhibit tumorigenesis and enhance immune function...

Claims

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