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Culture method of seaweed for zooplankton bait

A zooplankton and culture method technology, applied in the field of seaweed culture, can solve the problems of unfavorable absorption by aquaculture animals, insufficient stability of astaxanthin structure, and low degree of esterification, so as to enhance the body's immunity, shorten the time of synthesis and accumulation Cycle, low cost effect

Active Publication Date: 2019-03-22
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the structure of astaxanthin in Haematococcus pluvialis cultivated by this invention is not stable enough, such as the degree of esterification is low, which is not conducive to the absorption of aquaculture animals.

Method used

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  • Culture method of seaweed for zooplankton bait

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A method for cultivating Haematococcus pluvialis, specifically comprising the following steps:

[0029](1) Pretreatment of domestic sewage: standing for sedimentation, impurities and large particles in the sedimentation water; adding fruit peels to the filtered clarified liquid, first stirring for 5 minutes, then standing for 20 minutes; then filtering through a 25 micron membrane filter; filtering the filtered water Samples were sterilized by heating and boiling at 120°C for 20 minutes;

[0030] Above-mentioned pericarp is processed as follows: take the pericarp of pomelo, pomegranate, banana, mangosteen fruit, and this pericarp is the pericarp of growing green period; Take fresh pericarp, scrape off the wax on the surface with a knife, and then immerse in a temperature of 30 ℃. After soaking in 1.5M sodium polyacrylate solution for 15 minutes, wash it twice with 2M ethyl acetate solution and dry it in the air; the pores on the surface of the peel treated with sodium p...

Embodiment 2

[0034] A method for cultivating Haematococcus pluvialis, specifically comprising the following steps:

[0035] (1) Pretreatment of domestic sewage: standing for sedimentation, impurities and large particles in the sedimentation water; adding fruit peels to the filtered clarified liquid, first stirring for 8 minutes, then standing for 25 minutes; then filtering through a 20 micron filter membrane; filtering the filtered water The sample is sterilized by autoclaving: the sterilization temperature is 115°C, the sterilization pressure is 0.08MPa, and the sterilization time is 18min;

[0036] Above-mentioned pericarp is processed as follows: take the pericarp of pomelo, pomegranate, banana, mangosteen fruit, and this pericarp is the pericarp of growing green period; Get fresh pericarp, scrape off the wax on the surface with a knife, then immerse in a temperature of 33 ℃. Soak in 2.5M sodium polyacrylate solution for 20 minutes, wash with 2M ethyl acetate solution for 3 times, and d...

Embodiment 3

[0040] Utilize the obtained Haematococcus pluvialis cultured in Example 2 of the present invention to carry out zooplankton (rotifer) cultivation, rotifers are inoculated with a density of 20 / ml in the filtered pond water, and the rotifer culture solution is grown at 26° C. Keep ventilated down, and feed the gained Haematococcus pluvialis (living cell) that the present invention cultivates, cultivate 7 days, can make the density proliferation of rotifers to 2300 / ml. Microalgae can also be fed with other baits; the amount of feeding is adjusted according to the density of zooplankton.

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Abstract

The invention provides a culture method of seaweed for zooplankton bait. Domestic wastewater is used as a raw material for culturing alga and astaxanthin is synthesized by illuminating induction of alga through low molecular weight organic matters. The culture method of the alga comprises the following steps: adding small molecule unsaturated fatty acid into a domestic wastewater culture medium and culturing at the illumination intensity of 3000 to 4500 Lux; after microalgae cells turn brown under illuminating induction culture, adjusting the components of the culture medium to contain indolesubstances and small molecular ester organic compounds without the small molecular unsaturated fatty acid; continuously culturing at the light intensity of 1000 to 3500 Lux. By taking the domestic wastewater as the culturing raw material of the alga, the method provided by the invention is green and environment-friendly and low in cost; the astaxanthin is short in synthesis period and high in content; the algae rich in the astaxanthin is used as the bait for zooplanktons and further is applied to aquaculture animals, so that the transmission effect of a food chain is realized.

Description

technical field [0001] The invention belongs to the field of bait for cultivating zooplankton used by aquaculture animals, and in particular relates to a method for cultivating seaweed used as bait for zooplankton. Background technique [0002] Fish farming has increased in recent years as fish stocks have dwindled worldwide and health awareness has increased. There are roughly two farming methods: the method of catching natural juvenile fish and feeding them with mixed feed and raising them to a marketable size, and the method of taking eggs from broodstock and raising them after hatching. In the latter case, juveniles grow on a nutrient substance called yolk for a certain period after hatching, after which they open their mouths and start feeding, but to promote good growth, live zooplankton such as rotifers and daphnia are usually used. [0003] Astaxanthin (also known as astaxanthin or astaxanthin) is a kind of carotenoid and a strong natural antioxidant. The antioxida...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 张建设景斐丁月晗
Owner ZHEJIANG OCEAN UNIV
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