Construction and application of astaxanthin synthesis strain

A technology of astaxanthin and recombinant bacteria, applied in the directions of enzymes, bacteria, isomerases, etc., can solve the problems that the protein cannot be folded correctly, limit the production of astaxanthin, and it is difficult to produce high yields, so as to eliminate the toxicity of metabolic intermediates, increase the Astaxanthin production, the effect of increasing the production capacity
CN112852694AActive Publication Date: 2021-05-28TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Publication Date
2021-05-28

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Abstract

The invention discloses construction and an application of an astaxanthin synthesis strain. The invention provides a recombinant bacterium, which is prepared according to a method comprising the following steps of: expressing and synthesizing carotenoid related genes crtY gene, crtZ gene and crtW gene in escherichia coli, and regulating and controlling the expression of molecular chaperone genes groES and groEL in the escherichia coli to obtain the recombinant bacterium, according to the invention, the promoters with different intensities are utilized and gene copy is changed, so that coordinated expression among genes in a synthetic pathway of astaxanthin is realized, and the toxicity of metabolic intermediates is eliminated, so that the generation capacity of biosynthesis of astaxanthin is improved. In addition, stabilizing the expression of the exogenous gene and increasing the correct folding of the exogenous gene can also become an effective way for improving the biological activity of astaxanthin synthetic pathway enzyme and increasing the yield of astaxanthin.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to the construction and application of astaxanthin-synthesizing bacterial strains. Background technique

[0002] Astaxanthin (3,3'-dihydroxy-β,β'-carotene-4,4'-diketonyl) is a high-value ketocarotenoid with strong antioxidant and mono Heavy oxygen quenching activity can regulate biological functions ranging from lipid peroxidation to tissue resistance to UV damage. Of the more than 600 known natural carotenoids, beta-carotene, lutein, lycopene, zeaxanthin, and astaxanthin, the most powerful antioxidant known , which is about 100 times more potent than other carotenoids such as beta-carotene, lutein and vitamin E. Thus, astaxanthin is able to protect cells, lipids and membrane lipoproteins from oxidative damage. In addition, astaxanthin has a variety of other biological properties, including anti-inflammatory, anti-cancer, and immune system-boosting properties. As an appr...

Claims

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