Method for culturing haematococcus pluvialis and application of haematococcus pluvialis

A technology of Haematococcus pluvialis and culture medium, which is applied in the field of microorganisms, can solve the problem that the cost performance is not optimal, and achieve the effects of reducing nitrogen and phosphorus, good development prospects and increasing biomass.

Inactive Publication Date: 2016-04-13
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In order to obtain high-density biomass, a large number of studies have tried to optimize various media, including nutrient element content and composition, etc.; however, the cost performance is still not optimal, and there is a contradiction between yield and cost

Method used

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  • Method for culturing haematococcus pluvialis and application of haematococcus pluvialis
  • Method for culturing haematococcus pluvialis and application of haematococcus pluvialis
  • Method for culturing haematococcus pluvialis and application of haematococcus pluvialis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The sewage is taken from the aerated grit chamber of Xili Reclaimed Water Plant of Shenzhen Water Group, Nanshan District, Shenzhen City, Guangdong Province.

[0047] After the water sample was brought back to the laboratory, it was left to settle to settle impurities and large particles in the water; then it was filtered with a filter membrane with a pore size of 10 μm to remove fine particle impurities and plankton; finally, it was sterilized at 121°C and 0.1MPa for 15 minutes at high temperature .

[0048] The nitrogen and phosphorus content and total nitrogen and total phosphorus content of the sewage treated before cultivation were determined by automatic intermittent chemical analyzers (AutoDiscrete Analyzers, DeChem-TechCleverchem 380, Germany). Determination results are as follows: ammonia nitrogen (NH 4 + )8.6767mg / L, nitrous nitrogen (NO 2 - )0.0063mg / L, nitrate nitrogen (NO 3 - ) 0.0421mg / L, phosphate (PO 4 3- ) 0.983mg / L, total nitrogen (TN) 14.2643m...

Embodiment 2

[0050] 1. The cultivation of algae cells under different nutritional conditions

[0051] The algal species used in the experiment was Haematococcus pluvialis, the initial inoculation density was 5000 / mL, and they were divided into 15 250mL conical flasks (150mL for each bottle). groups of 3 in parallel.

[0052] The cultivation conditions of the algae are as follows: the temperature is 25±1° C., the light time L:D=12h:12h, and the light intensity is 3300±200 Lux. Shake once a day, and switch locations to ensure each receives the same amount of light.

[0053] The five experimental groups are as follows:

[0054] Control group: culture Haematococcus pluvialis with traditional BG11 culture medium;

[0055] Sewage undiluted group: the treated sewage was directly used as a culture medium to cultivate Haematococcus pluvialis;

[0056] Sewage diluted 2-fold group: the treated sewage was diluted 2-fold and used as a culture medium to cultivate Haematococcus pluvialis;

[0057] S...

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Abstract

The invention discloses a method I for culturing haematococcus pluvialis, a method II for improving biomass of haematococcus pluvialis, a method III for producing astaxanthin and a method IV for purifying domestic sewage. Through utilization of the method II, the biomass of haematococcus pluvialis can be greatly increased, haematococcus pluvialis can relatively quickly enter the astaxanthin synthesis period, the astaxanthin synthesis period can be greatly shortened, and good basis can be provided for follow-up increase of metabolite astaxanthin yield. Besides, the content of heavy metal and other pollutants in haematococcus pluvialis obtained by utilizing the method I is not obviously increased, so that the haematococcus pluvialis obtained by utilizing the method I can be used as a safe product for follow-up use. Meanwhile, through utilization of the method IV, the nitrogen content and the phosphorus content in sewage can be obviously reduced, and the sewage eutrophication degree is obviously relieved.

Description

technical field [0001] The present invention relates to the field of microorganisms, specifically, the present invention relates to Haematococcus pluvialls, its cultivation method and application, more specifically, the present invention relates to a method for cultivating Haematococcus pluvialls, a method for improving the quality of Haematococcus pluvialls A method of biomass, a method of producing astaxanthin and a method of purifying domestic sewage. Background technique [0002] Carotenoids have obvious antioxidant capacity due to their ability to resist singlet oxygen and free radical scavenging, and have shown significant effects in delaying and preventing atherosclerosis, cancer, aging and eye diseases. Astaxanthin is an orange-red carotenoid, which has attracted widespread attention due to its stronger antioxidant capacity and economic value. It exists in many aquatic organisms in nature, such as shrimp, crab and salmon. [0003] The antioxidant capacity of astaxa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P23/00C02F9/14C12R1/89
CPCC02F1/00C02F1/001C02F1/02C02F1/44C02F1/52C02F9/00C02F2301/08C12N1/12C12P23/00
Inventor 蔡中华张怀瑾周进张晶晶晋慧
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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