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Method for inducing algal cells to synthesize astaxanthin efficiently

A technology of algal cells and astaxanthin is applied in the field of inducing algal cells to efficiently synthesize astaxanthin, which can solve the problems of low astaxanthin content, high extraction cost, unsuitable for large-scale production, etc. Effects of the PP pathway and the TCA cycle

Active Publication Date: 2019-04-02
云南维他源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the content of astaxanthin in the waste is low, and the extraction cost is high, so it is not suitable for large-scale production

Method used

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  • Method for inducing algal cells to synthesize astaxanthin efficiently

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The method for inducing algae cells to efficiently synthesize astaxanthin specifically comprises the following steps:

[0037] Concentrated algae liquid: Collect the high-yield astaxanthin algae liquid of Haematococcus pluvialis in the logarithmic growth phase and conduct static sedimentation, remove the supernatant culture liquid, settle and remove impurities until the concentrated algae liquid with a cell density of 25g / L is obtained ; Selecting high-yield astaxanthin Haematococcus pluvialis in the logarithmic growth phase for screening and concentration is conducive to obtaining high-quality and efficient algae liquid, and achieving the most efficient induction of astaxanthin synthesis;

[0038] Cryopreservation: Equipped with cryopreservation solution: containing 0.1wt% 1-butanol and 0.05wt‰ L-arabitol, and the rest is distilled water; at 1°C, add the cryopreservation solution to the Concentrate the algae liquid, stir and centrifuge to remove the supernatant to obta...

Embodiment 2

[0045] A method for inducing algae cells to efficiently synthesize astaxanthin, comprising:

[0046] a) Settling and concentrating the high-yield astaxanthin Haematococcus pluvialis liquid in the logarithmic growth phase;

[0047] b) After adding the freezing liquid to the concentrated algae liquid, remove the supernatant, pre-freeze and then freeze for preservation;

[0048] c) Under the blue light irradiation of 440nm, induce and cultivate the algae liquid;

[0049] d) Adding fermentation aids to the carbon-free combined medium containing glucose to carry out shaking fermentation culture.

[0050] The method of the present invention freezes and preserves the high-yield astaxanthin-producing Haematococcus pluvialis algae liquid with the cryopreservation solution, which can reduce the growth rate of ice crystals and weaken the damage to algal cells caused by the extension of ice crystals in freezing overshoot, thereby maintaining the activity of algal cells , blue light irra...

Embodiment 3

[0064] A method for inducing algae cells to efficiently synthesize astaxanthin, comprising:

[0065] a) Settling and concentrating the high-yield astaxanthin Haematococcus pluvialis liquid in the logarithmic growth phase;

[0066] b) After adding the freezing liquid to the concentrated algae liquid, remove the supernatant, pre-freeze and then freeze for preservation;

[0067] c) Under the irradiation of blue light of 420-440nm, the algae liquid is induced to be cultured;

[0068] d) Adding fermentation aids to the carbon-free combined medium containing glucose to carry out shaking fermentation culture.

[0069] The method for inducing algae cells to efficiently synthesize astaxanthin specifically includes the following steps:

[0070] Concentrated algae liquid: Collect the high-yielding astaxanthin Haematococcus pluvialis liquid in the logarithmic growth phase and conduct static sedimentation, remove the supernatant culture liquid, settle and remove impurities until the conc...

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Abstract

The invention provides a method for inducing algal cells to synthesize astaxanthin efficiently, and belongs to the technical field of astaxanthin synthesis, wherein the method includes the steps: a) settling and concentrating a high-yielding astaxanthin algae solution at a logarithmic growth stage; b) adding a cryopreservation solution to the concentrated algae solution, removing a supernatant, and rapidly freezing and storing after pre-freezing; c) inducing to culture an algae solution under irradiation of 420-440 nm blue light; and d) adding a fermentation auxiliary agent to a carbon source-free combination culture medium containing glucose, and carrying out vibration fermentation culture. The method has the beneficial effects: the method can help to reduce the damage of algae cells in the process of frozen storage of the algae solution; firstly, the morphological changes of the cells are induced by specific-wavelength blue light irradiation in the process of culture, and the synthesis of astaxanthin is promoted; then, the auxiliary agent is added in the fermentation process to increase the flux of pyruvic acid and acetyl CoA, a PP pathway and the TCA circulation are strengthened, and the metabolism synthesis of astaxanthin is improved.

Description

technical field [0001] The invention belongs to the technical field of astaxanthin synthesis, and in particular relates to a method for inducing algae cells to efficiently synthesize astaxanthin. Background technique [0002] Astaxanthin, also known as astaxanthin and lobster shell pigment, is a carotenoid and the highest grade product of carotenoid synthesis. It is a fine dark purple-brown powder with a melting point of 216°C and a boiling point of 774°C. Density 1.071g / cm3, not easily soluble in water, easily soluble in acetone, ethanol, dichloromethane, ether, n-hexane and other organic solvents, its chemical structure is similar to β-carotene. Chemical name: 3,3′-dihydroxy-4,4′-diketo-β-carotene, pigment Aj067-69, CAS No:472-61-7, molecular formula C 40 h 52 o 4 , the molecular weight is 596.86. The astaxanthin molecule is composed of 4 isoprene double bonds connected at the first position, with a total of 11 conjugated double bonds, which is a typical carotenoid car...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12R1/89
CPCC12P23/00
Inventor 景斐丁月晗曾善美
Owner 云南维他源生物科技有限公司
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