A method for improving the growth rate and protein content of microalgae by using silk reeling wastewater

A technology for protein content and silk reeling wastewater, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of blackened and smelly rivers, environmental pollution, etc., to promote growth, reduce production costs, and realize The effect of resource utilization

Active Publication Date: 2022-04-15
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

If the silk reeling wastewater is directly discharged into the river, the river will be rapidly eutrophicated in a short period of time, causing the river to become black and smelly, causing serious environmental pollution
[0004] However, there is currently no case where silk reeling wastewater is used as a nutrient source to promote the growth rate of microalgae and increase its protein content, thereby realizing the resource utilization of silk reeling wastewater

Method used

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  • A method for improving the growth rate and protein content of microalgae by using silk reeling wastewater
  • A method for improving the growth rate and protein content of microalgae by using silk reeling wastewater
  • A method for improving the growth rate and protein content of microalgae by using silk reeling wastewater

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Embodiment 1

[0026] The present embodiment further elaborates the present invention according to the following steps:

[0027] (1) Using Chlorella sp. as the microalgae species, first use BG11 culture medium for enrichment culture, when the cell density of Chlorella sp. reaches 1×10 8 ~9×10 8 cells / mL, the Chlorella seed solution was obtained. The composition of BG11 culture medium is: NaNO 3 (1.5g / L), K 2 HPO 4 (40mg / L), MgSO 4 ·7H 2 O (70mg / L), CaCl 2 2H 2 O (40mg / L), citric acid (6mg / L), ferric ammonium citrate (6mg / L), EDTANa 2 (1mg / L), Na 2 CO 3 (20mg / L), H 3 BO 3 (2.86mg / L), MnCl 2 4H 2 O (1.86mg / L), ZnSO 4 ·7H 2 O (0.22mg / L), Na 2 MoO 4 2H 2 O (0.39mg / L), CuSO 4 ·5H 2 O(0.08mg / L), Co(NO 3 ) 2 ·6H 2 O (0.05mg / L), pH=7.00.

[0028] (2) diluting the silk reeling wastewater with water to form the silk reeling wastewater with a concentration gradient of 10-50%. The chlorella seed solution obtained in step (1) was inoculated into the silk reeling wastewater of di...

Embodiment 2

[0032] The present embodiment further elaborates the present invention according to the following steps:

[0033](1) Using Scenedesmus obliquus as the microalgae species, first use BG11 culture medium for enrichment culture, when the cell density of Scenedesmus obliquus reaches 1×10 8 ~9×10 8 cells / mL, the seed liquid of Scenedesmus obliquus was obtained. The composition of the BG11 culture solution is the same as in Example 1.

[0034] (2) diluting the silk reeling wastewater with water to form the silk reeling wastewater with a concentration gradient of 10-50%. Inoculate the Scenedesmus obliquus seed solution obtained in step (1) into the silk reeling wastewater of different concentrations according to the volume ratio of 1.5%, forming an initial cell density of 1 × 10 6 ~9×10 6 cells / mL culture of Scenedesmus obliquus.

[0035] (3) Place the culture of Scenedesmus obliquus obtained in step (2) on a light shaker for 24 days, and the culture conditions are: temperature 2...

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Abstract

The invention discloses a method for increasing the growth rate and protein content of microalgae by using silk reeling wastewater, which mainly includes the following steps: (1) placing the microalgae species in BG11 culture solution for enrichment culture, when the cell density of microalgae up to 1×10 8 ~9×10 8 At the time of 1 cell / mL, the microalgae seed liquid is obtained; (2) the microalgae seed liquid obtained in step (1) is inoculated into silk reeling wastewater of different concentrations according to the volume ratio of 0.8 to 1.5%, to form a microalgae culture; (3) Place the microalgae culture prepared in the step (2) on a light shaker and culture it for 16-24 days. The method provided by the invention can not only significantly increase the growth rate of the microalgae cells, but also significantly increase the protein content in the microalgae cells. In addition, the invention can significantly reduce the production cost of microalgae large-scale cultivation, and is easy to realize industrial operation.

Description

technical field [0001] The invention belongs to the technical field of microalgae biology, and in particular relates to a method for increasing the growth rate and protein content of microalgae by utilizing silk reeling wastewater. Background technique [0002] Microalgae is a general term for a class of single-celled photosynthetic micro organisms. Most microalgae live in water. They are simple in structure and tiny and can only be observed under a microscope. Their cells are rich in polysaccharides, proteins, pigments, unsaturated fatty acids, terpenoids, vitamins and minerals, etc., which are harmful to the human body. Healthy and beneficial bioactive substances with high nutritional value are natural nutritional and health food. In addition, microalgae have the basic characteristics of a wide variety of species, a large population, and rapid growth and reproduction. They have important development significance and research value in solving problems such as food, health,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12P21/00C02F3/32C12R1/89
CPCC12N1/12C12P21/00C02F3/32
Inventor 高坤邓祥元王迪李达汪羚胡小丽
Owner JIANGSU UNIV OF SCI & TECH
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