Method for producing ginsenosides by cell suspension culture method

A technology of ginsenoside and cell suspension, which is applied in the direction of cell culture active agents, biochemical equipment and methods, plant cells, etc. It can solve the problems that the amount of ginsenosides cannot meet the market demand, the purity is not high, and the yield is low, so as to improve the yield. efficiency, improve purity, and promote growth

Active Publication Date: 2019-02-05
江西中天医药生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the demand for ginsenosides continues to increase, the amount of ginsenosides grown naturally and artificially cannot meet market demand.
[0003] At present, there is also a cell suspension culture method to produce ginsenosides, but the purity is not high and the yield is low

Method used

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  • Method for producing ginsenosides by cell suspension culture method
  • Method for producing ginsenosides by cell suspension culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The method for producing ginsenosides by cell suspension culture method, the specific steps are:

[0042] 1. Ginseng tissue cell collection

[0043] (1) Take fresh ginseng roots and whiskers that have been grown for more than 3 years, wash them, soak them in 75% ethanol for 5 minutes, then soak them in 2% sodium hypochlorite solution for 30 minutes, and wash them with sterile water until there is no sodium hypochlorite residue;

[0044] (2) Cut the ginseng roots and hairs after cleaning and disinfection into pieces, put them into a beater, add nutrient solution, add 20ml nutrient solution for every gram of ginseng block, and beat;

[0045] Culture medium composition: MS medium 10g / L, 6-benzylaminopurine 1mg / L, yeast dextran 0.8mg / L, glucose 20g / L;

[0046] (3) After beating, filter at 60 mesh, collect the filtrate, add the filtrate to a 250ml Erlenmeyer flask, 100-150ml per bottle, put the Erlenmeyer flask on a shaker for cultivation, the speed is 30r / min, the temperat...

Embodiment 2

[0057] The method for producing ginsenosides by cell suspension culture method, the specific steps are:

[0058] 1. Ginseng tissue cell collection

[0059] (1) Take fresh ginseng roots and whiskers that have been grown for more than 3 years, wash them, soak them in 75% ethanol for 7 minutes, then soak them in 3% sodium hypochlorite solution for 30 minutes, and wash them with sterile water until there is no sodium hypochlorite residue;

[0060] (2) Cut the ginseng roots and hairs after cleaning and disinfection into pieces, put them into a beater, add nutrient solution, add 20ml nutrient solution for every gram of ginseng block, and beat;

[0061] Culture medium composition: MS medium 10g / L, 6-benzylaminopurine 1mg / L, yeast dextran 0.8mg / L, glucose 20g / L;

[0062] (3) After beating, filter at 60 mesh, collect the filtrate, add the filtrate to a 250ml Erlenmeyer flask, 100-150ml per bottle, put the Erlenmeyer flask on a shaker for cultivation, the rotation speed is 40r / min, the t...

Embodiment 3

[0073] The method for producing ginsenosides by cell suspension culture method, the specific steps are:

[0074] 1. Ginseng tissue cell collection

[0075] (1) Take fresh ginseng roots and whiskers that have been grown for more than 3 years, wash them, soak them in 75% ethanol for 10 minutes, then soak them in 4% sodium hypochlorite solution for 30 minutes, and wash them with sterile water until there is no sodium hypochlorite residue;

[0076] (2) Cut the ginseng roots and hairs after cleaning and disinfection into pieces, put them into a beater, add nutrient solution, add 20ml nutrient solution for every gram of ginseng block, and beat;

[0077] Culture medium composition: MS medium 10g / L, 6-benzylaminopurine 1mg / L, yeast dextran 0.8mg / L, glucose 20g / L;

[0078] (3) After beating, filter at 60 mesh, collect the filtrate, add the filtrate to a 250ml Erlenmeyer flask, 100-150ml per bottle, put the Erlenmeyer flask on a shaker for cultivation, the rotation speed is 50r / min, th...

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Abstract

The invention discloses a method for producing panaxoside by adopting a cell suspension culture method. The method comprises the following steps: inducing ginseng callus cell proliferation and fission, performing cell suspension culture, adding plant modifier 6-benayl aminopurine into a culture medium, and then adding yeast glucan, citronellol and quercetin as additives and adopting a certain technological parameter and synergistic effect, thereby acquiring the panaxoside with high yield and purity.

Description

technical field [0001] The production technology related to ginsenoside of the present invention relates to the method for producing ginsenoside by cell suspension culture method in particular. Background technique [0002] Ginsenoside is the main component of ginseng, widely used in food, health products, medicine, chemical industry and other fields. Modern medicine believes that ginsenosides can improve human immunity and delay aging. The traditional extraction method of ginsenosides is obtained by extracting the roots, stems, leaves, fibrous roots and other plants of perennial ginseng plants. With the increasing demand for ginsenosides, the quantity of ginsenosides grown naturally and artificially cannot meet the market demand more and more. [0003] At present, there is also a cell suspension culture method to produce ginsenosides, but the purity is not high and the yield is low. Contents of the invention [0004] The object of the present invention is to provide a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/20C12N5/04C12N5/02C07G3/00
CPCC07G3/00C12N5/04C12N2500/40C12N2501/90C12N2501/999C12P33/20
Inventor 高国文李成林
Owner 江西中天医药生物有限公司
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