Method for inducing algae cells to efficiently synthesize astaxanthin

Abstract

The invention provides a method for inducing algae cells to efficiently synthesize astaxanthin, which belongs to the technical field of astaxanthin synthesis, comprising: a) settling and concentrating high-yield astaxanthin algae liquid in the logarithmic growth phase; b) adding to the concentrated algae liquid After freezing the solution, remove the supernatant, pre-freeze and quickly freeze and store it; c) Induce the cultivation of the algae liquid under 420-440nm blue light irradiation; d) Add fermentation aids to the carbon-free combined medium containing glucose for shaking fermentation nourish. The beneficial effect is: the method helps to reduce the damage to the algae cells during the frozen storage of the algae liquid. Firstly, the blue light of a specific wavelength is irradiated during the culture process to induce the change of the cell shape and promote the synthesis of astaxanthin, and then During the fermentation process, additives were added to increase the flux of pyruvate and acetyl CoA, strengthen the PP pathway and TCA cycle, and improve the metabolic synthesis of astaxanthin.

Description

technical field

[0001] The invention belongs to the technical field of astaxanthin synthesis, and in particular relates to a method for inducing algae cells to efficiently synthesize astaxanthin. Background technique

[0002] Astaxanthin, also known as astaxanthin and lobster shell pigment, is a carotenoid and the highest grade product of carotenoid synthesis. It is a fine dark purple-brown powder with a melting point of 216°C and a boiling point of 774°C. Density 1.071g / cm3, not easily soluble in water, easily soluble in acetone, ethanol, dichloromethane, ether, n-hexane and other organic solvents, its chemical structure is similar to β-carotene. Chemical name: 3,3′-dihydroxy-4,4′-diketo-β-carotene, pigment Aj067-69, CAS No:472-61-7, molecular formula C 40 h 52 o 4 , the molecular weight is 596.86. The astaxanthin molecule is composed of 4 isoprene double bonds connected at the first position, with a total of 11 conjugated double bonds, which is a typical carotenoid car...

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