Research method for synthetic process of haematococcus pluvialis astaxanthin based on infrared spectroscopy microscopic imaging technology

A technology of Haematococcus pluvialis and infrared spectroscopy, applied in the field of research on the synthesis process of astaxanthin from Haematococcus pluvialls, can solve the problems of single component, cumbersome steps, environmental pollution, etc., achieve low cost, simple steps, and analytical short time effect

Inactive Publication Date: 2018-11-30
ANHUI SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method has the disadvantages of complex operation, cumbersome steps, the use of a large number of chemical reagents in the detection process,

Method used

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  • Research method for synthetic process of haematococcus pluvialis astaxanthin based on infrared spectroscopy microscopic imaging technology
  • Research method for synthetic process of haematococcus pluvialis astaxanthin based on infrared spectroscopy microscopic imaging technology
  • Research method for synthetic process of haematococcus pluvialis astaxanthin based on infrared spectroscopy microscopic imaging technology

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Embodiment 1

[0032] A method for studying the synthetic process of Haematococcus pluvialis astaxanthin based on infrared spectrum microscopic imaging technology, comprising the following steps:

[0033] S1. Inoculate Haematococcus pluvialis into sterile BBM medium at a weight ratio of 10%, under the conditions of light intensity 2000lx, temperature 23°C, light-dark cycle 12h / 12h, and hand frequency 3 times / day Cultivate and obtain Haematococcus pluvialis strain;

[0034] S2. Adding nicotine to the culture medium of Haematococcus pluvialis to prepare a treatment solution containing nicotine at a concentration of 60 μM, and cultivating it for 2 days, 5 days and 10 days under a strong light condition of 5500 lx to induce the accumulation of astaxanthin;

[0035] S3. The astaxanthin, β-carotene and lycopene samples were scanned 64 times by the infrared spectrometer respectively, and the measurement range was 4000-400cm -1 , resolution 4cm -1 The infrared spectrum was measured under the condi...

Embodiment 2

[0039] A method for studying the synthetic process of Haematococcus pluvialis astaxanthin based on infrared spectrum microscopic imaging technology, comprising the following steps:

[0040] S1. Inoculate Haematococcus pluvialis in a sterile BBM medium at a weight ratio of 20%, under the conditions of light intensity 3000lx, temperature 26°C, light-dark cycle 12h / 12h, and hand shaking frequency 3 times / day Cultivate and obtain Haematococcus pluvialis strain;

[0041] S2. Adding nicotine to the culture medium of Haematococcus pluvialis to prepare a treatment solution containing nicotine at a concentration of 90 μM, and cultivating it for 2 days, 5 days and 10 days under strong light conditions of 6500 lx to induce the accumulation of astaxanthin;

[0042] S3. The astaxanthin, β-carotene and lycopene samples were scanned 64 times by the infrared spectrometer respectively, and the measurement range was 4000-400cm -1 , resolution 4cm -1 The infrared spectrum was measured under th...

Embodiment 3

[0046] A method for studying the synthetic process of Haematococcus pluvialis astaxanthin based on infrared spectrum microscopic imaging technology, comprising the following steps:

[0047] S1. Inoculate Haematococcus pluvialis into sterile BBM medium at a weight ratio of 10%, under the conditions of light intensity 2000lx, temperature 25°C, light-dark cycle 12h / 12h, and hand frequency 3 times / day Cultivate and obtain Haematococcus pluvialis strain;

[0048] S2. Adding diphenylamine to the culture medium of Haematococcus pluvialis to prepare a treatment solution containing diphenylamine at a concentration of 0.2mM, and cultivating it for 2 days, 5 days and 10 days under strong light conditions of 6000lx to induce astaxanthin accumulation;

[0049] S3. The astaxanthin, β-carotene and lycopene samples were scanned 64 times by the infrared spectrometer respectively, and the measurement range was 4000-400cm -1 , resolution 4cm -1 The infrared spectrum was measured under the con...

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Abstract

The invention discloses a research method for the synthetic process of haematococcus pluvialis astaxanthin based on an infrared spectroscopy microscopic imaging technology. The method comprises the following steps that inoculation and culture are carried out on haematococcus pluvialis; an inhibitor performs treatment on haematococcus pluvialis strains; the infrared spectroscopy of the astaxanthin,beta-carotene and lycopene are collected; the infrared microscopic spectroscopy of haematococcus pluvialis cells are detected; and comparison and analysis of imaging of the microscopic infrared spectroscopy are carried out. According to the method, the infrared microscopic spectroscopy imaging technology is utilized, the haematococcus pluvialis cells are treated by combining the inhibitor synthesized by carotenoid, and high-spatial-resolution in-situ observation is carried out on the contents of various components of the haematococcus pluvialis cells in the astaxanthi accumulation process, sothat the effects of the components in the synthesis path of the astaxanthin can be conveniently analyzed. The method has important significance in research of the synthesis path of the carotenoid inthe haematococcus pluvialis cells and large-scale breeding of the haematococcus pluvialis for production of the astaxanthin.

Description

technical field [0001] The invention relates to the technical field of microalgae metabolic process research, in particular to a research method for the synthesis process of Haematococcus pluvialis astaxanthin based on infrared spectrum microscopic imaging technology. Background technique [0002] Haematococcus pluvialis is a freshwater unicellular green alga belonging to Chlorophyta, Volvox, Haematococcus, and Haematococcus. Cells are round to oval in shape, 20-40 μm in diameter. During the whole life cycle, it presents three typical cell forms: green vegetative cells, yellow metaphase cells and red cyst cells. Under suitable growth conditions, Haematococcus pluvialis grows rapidly in the form of green swimming cells. At this time, the cells mainly contain chlorophyll a, chlorophyll b and primary carotenoids, especially β-carotene and lutein. white. Under unfavorable environmental conditions (such as strong light, high salt, nutrient deficiency, low temperature, dryness,...

Claims

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Application Information

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IPC IPC(8): G01N21/3563
CPCG01N21/3563
Inventor 刘京华陈心怡
Owner ANHUI SCI & TECH UNIV
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