Method for preparing water-soluble astaxanthin and astaxanthin aqueous solution prepared by method

Abstract

The invention provides a method for preparing water-soluble astaxanthin. The method comprises the following steps: mixing astaxanthin-containing raw materials with an organic acid-containing solutionand then carrying out cell disruption;leaching, wherein natural astaxanthin can interact with naturally existing components such as protein, nucleic acid or polysaccharide in the raw materials, so that the water-soluble astaxanthin is directly prepared, astaxanthin does not need to be modified, and the astaxanthin is natural in source, simple in operation process, low in equipment requirement, lowin production cost, environmentally friendly, safe, free of organic solvent residues and easy to industrialize.

Description

technical field [0001] The invention relates to the field of biochemical technology, in particular to a method for preparing water-soluble astaxanthin and an aqueous solution of astaxanthin prepared therefrom. Background technique [0002] Astaxanthin has physiological activities such as protecting the skin from light damage, preventing arteriosclerosis and related diseases, anti-cancer activity, enhancing the function of the immune system, maintaining the health of the eyes and central nervous system, and anti-infection. It is widely used in food, medicine, cosmetics and aquaculture Industry and other fields have a wide range of applications, so the related astaxanthin extraction and application technology is attracting more and more people's attention. [0003] In the current patent and related literature research, the techniques for extracting astaxanthin mainly include: organic solvent extraction, supercritical CO 2 Extraction method, enzymatic hydrolysis method and mic...

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Problems solved by technology

[0004] CN110724083A discloses a kind of organic solvent extraction method, because astaxanthin structure is unstable during extraction, isomerization reaction may occur and reduce its antioxidant activity

Moreover, most of the organic solvents used in this type of organic solvent extraction have certain toxicity, and there will be reagent residue problems after extraction, which limits the application of the product in the food industry

[0005] CN109608376A discloses a supercritical CO 2 Extraction method, but this method needs to have a pressure resistance capacity of dozens of atmospheres in production applications, high equipment requirements, large capital investment, high production costs, and this technology involves the operation of high-pressure resistant equipment, which requires high production technology. It also limits its application in industrial production

[0006] CN107805215B discloses a method for extracting astaxanthin by enzymatic hydrolysis. Although the enzymatic hydrolysis method is used to break the wall of Haematococcus pluvialis for the second time in the extraction process, which improves the extraction rate of astaxanthin, the reaction does not affect temperature, pH and The operating conditions such as time are relatively high, and the operation process is complicated, and the use of enzyme preparations also increases the production cost

[0007] In 2007, Wang Lingzhao et al. used microwave method to assist in the organic extraction of astaxanthin (see "Technology Research on Extracting Astaxanthin from Haematococcus Pluvialls by Microwave Method", Wang Lingzhao et al., Food Research and Development, Volume 28, Issue 12 , pages 96-100), although the extraction rate has been improved, the temperature rise in the microwave extraction process will cause the oxidative decomposition of astaxanthin, and the selectivity of microwave extraction is relatively poor, which is not conducive to the separation of astaxanthin

[0010] CN108403666A discloses a microencapsulation method. Although the method can improve the stability and physical properties of biomolecules, there are problems such as discontinuous operation, which is not conducive to linkage production, and the embedding is difficult to recycle, resulting in high preparation costs.

[0011] In 2013, Navideh A et al. improved the water solubility of astaxanthin by adding hydrophilic emulsifiers (polysorbate and sucrose ester). However, this technology relies on suitable emulsification homogenization means and drying technology, and at the same time, after emulsification and volatilization, residual Organic solvents in astaxanthin products will also cause certain biosafety problems (see "Effects of Selected Polysorbate and Sucrose Ester Emulsifiers on the Physicochemical Properties of Astaxanthin Nanodispersions", Navideh A et al., Molecules, No. 18, pages 768-777)

[0012] In 2007, Xiaolin Chen et al. used the clathrate method to clathrate astaxanthin in β-cyclodextrin (see "The preparation and stability of the inclusion complex of astaxanthin with β-cyclodextrin", Xiaolin Chen et al., Food Chemistry, No. 101 period, pages 1580-1584), although the solubility and stability of astaxanthin have been improved, the drug-loading capacity of this method is low, generally only 9% to 14%, which causes waste of resources and increases production costs, which is not conducive to Industrial production

[0013] CN109646425A discloses a method for constructing astaxanthin nanosystems with chitosan and DNA. Although the method is simple to operate and does not require high equipment, most of the components of polymer materials are obtained by chemical methods such as artificial synthesis, resulting in Polymer materials have some deficiencies in biocompatibility, degradability, cytotoxicity, and organic solvent residues.

[0014] In summary, the existing natural astaxanthin product preparation process often includes two steps: extraction technology and water-soluble astaxanthin preparation technology, and the various technologies involved in the two-step process have certain defects.

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