Synchronous enrichment method of lipide and protein in pandalus borealis processing by-products
A technology of arctic sweet shrimp and by-products, applied in protein food components, fish protein components, fat production, etc., can solve the problems of high-efficiency extraction of shrimp oil, inability to simultaneously enrich proteins, etc., and improve the comprehensive utilization rate , Reasonable amino acid composition and good processing properties
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Embodiment 1
[0051] A method for synchronously enriching lipids and proteins in arctic sweet shrimp processing by-products, comprising the steps of:
[0052] (1) Weigh 1 kg of arctic sweet shrimp heads, crush and mix them with a chopping machine until they are minced, add 2 kg of water during the chopping process; then add neutral protease into the system, Stirring and enzymatic hydrolysis for 2 hours, the amount of protease added is 500u / g arctic sweet shrimp head.
[0053] (2) Add 100 g of ethyl acetate to the above enzymatic hydrolysis system, and then use a microbubble generator to continuously input airflow into the system for about 1 hour, then filter and separate, and collect the crude extract.
[0054] (3) Build a multiphase separation system: first add dehydrated alcohol with a volume ratio of 1:2 to the crude extract obtained in step 2), stir fully, and mix well; after mixing, add a volume ratio of 1:2 n-hexane, fully stirred, and mixed evenly; after mixing, add ammonium sulfate...
Embodiment 2
[0060] A method for synchronously enriching lipids and proteins in arctic sweet shrimp processing by-products, comprising the steps of:
[0061] (1) Weigh 1 kg of arctic sweet shrimp heads and 1 kg of shrimp hairs, crush them with a tissue grinder until they are minced, add 5 kg of water during the crushing process; then add papain to the system, and heat the Stirring and enzymatic hydrolysis for 4 hours under the same conditions, the amount of protease added is 1000u / g (Arctic sweet shrimp shrimp head + shrimp hair).
[0062] (2) Add 300 g of oleic acid to the above enzymatic hydrolysis system, and then use a microbubble generator to continuously input airflow into the system for about 0.5 h, then centrifuge to collect the crude extract.
[0063] (3) Build a multiphase separation system: first add dehydrated alcohol with a volume ratio of 1:3 to the crude extract obtained in step 2), stir fully, and mix well; after mixing, add a volume ratio of 1:1 n-hexane, fully stirred, a...
Embodiment 3
[0069] A method for synchronously enriching lipids and proteins in arctic sweet shrimp processing by-products, comprising the steps of:
[0070] (1) Weigh 100kg of arctic sweet shrimp heads, crush and mix them with a chopping machine until they are minced, add 200kg of water during the chopping process; Stirring and enzymatic hydrolysis for 3 hours, the amount of protease added is 800u / g arctic sweet shrimp head.
[0071] (2) Add 15 kg of corn oil to the above enzymatic hydrolysis system, and then use a microbubble generator to continuously input airflow into the system for about 2 hours, then filter and separate, and collect the crude extract.
[0072] (3) Build a multiphase separation system: first add dehydrated alcohol with a volume ratio of 1:2 to the crude extract obtained in step 2), stir fully, and mix well; after mixing, add a volume ratio of 1: 0.7 n-hexane, fully stirred, mixed evenly; after mixing, add ammonium sulfate solution with a volume ratio of 1:0.5 to the ...
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