Method for preparing microbial cultures through solid state fermentation of shrimp shells

A technology of microbial culture and solid-state fermentation, applied in the fields of bacteria, food science, animal feed, etc. used in food preparation, can solve the problems of environmental pollution and high production costs, reduce production costs, reduce pollution, and increase the content of astaxanthin Effect

Pending Publication Date: 2019-01-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing methods of developing and utilizing shrimp shell resources are mainly to prepare chitin, antioxidant peptides and other products by hydrolyzing shrimp shells, fermenting, filtering, spraying and other processes, such as: Chinese Patent Application No. CN 105506039 A "Preparation of Shrimp Shells Fermented by Aspergillus oryzae Aeronautical Titanium Oxide," and Chinese Patent Application No. CN 103156049 A "A Method for Extracting Protein and Chitin by Fermenting Shrimp Heads and Shrimp Shells" disclose a method for preparing functional products from shrimp shells. High cost and will pollute the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Prepare the shrimp shell solid-state fermented feed material of this embodiment according to the following method:

[0023] (1) Shrimp shell steam explosion sterilization treatment: put the shrimp shell into the steam explosion tank, feed 2.1Mpa steam into the steam explosion tank, keep it for 2.5min, open the steam explosion valve, and obtain the shrimp shell after steam explosion;

[0024] (2) Dehydration: the tea leaves after steam explosion are extruded or dried by equipment, so that the moisture content is controlled at 55% by mass fraction;

[0025] (3) Fermentation substrate preparation: 71.97 parts by weight of dehydrated shrimp shells, 8 parts by weight of bran, 10 parts by weight of soybean meal, and 0.03 parts by weight of composite trace elements are fully mixed to make a fermentation substrate; the composite trace elements are made of 2 parts by weight part FeS0 4 ·7H 2 0, 1.0 parts by weight MnS0 4 , 0.8 parts by weight ZnS0 4 , 1.0 parts...

Embodiment 2

[0032] Embodiment 2: Prepare the shrimp shell solid-state fermented feed material of this embodiment according to the following method:

[0033] (1) Shrimp shell steam explosion sterilization treatment: put the shrimp shell into the steam explosion tank, feed 2.5Mpa steam into the steam explosion tank, keep it for 3min, open the steam explosion valve, and obtain the shrimp shell after steam explosion;

[0034] (2) Dehydration: the shrimp shells after steam explosion are extruded or dried by equipment, so that the water content is controlled at 50% by mass fraction;

[0035] (3) Fermentation substrate preparation: 68.96 parts by weight of dehydrated shrimp shells, 10 parts by weight of bran, 10 parts by weight of soybean meal, and 0.04 parts by weight of composite trace elements are fully mixed to make a fermentation substrate; the composite trace elements are made of 2 parts by weight part FeS0 4 ·7H 2 0, 0.9 parts by weight MnS0 4 , 0.8 parts by weight ZnS0 4 , 1.0 parts ...

Embodiment 3

[0042] Embodiment 3: The shrimp shell solid-state fermented feed material of this embodiment is prepared according to the following method:

[0043](1) Shrimp shell steam explosion sterilization treatment: put the shrimp shell into the steam explosion tank, feed 3Mpa steam into the steam explosion tank, keep it for 4min, open the steam explosion valve, and obtain the shrimp shell after steam explosion;

[0044] (2) Dehydration: the shrimp shells after steam explosion are extruded or dried by equipment, so that the water content is controlled at 55% by mass fraction;

[0045] (3) Preparation of fermentation substrate: 84 parts by weight of dehydrated shrimp shells, 10 parts by weight of bran, 15 parts by weight of soybean meal, and 0.05 parts by weight of composite trace elements are fully mixed to make a fermentation substrate; the composite trace elements are made of 2.3 parts by weight part FeS0 4 ·7H 2 0,0.8 parts by weight MnS0 4 , 0.9 parts by weight ZnS0 4 , 1.0 part...

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Abstract

The invention relates to a method for preparing microbial cultures through solid state fermentation of shrimp shells, and relates to the field of feed processing. The method comprises the following steps of taking shrimp shells, soybean meal, bran and composite trace elements in a certain mass ratio, performing mixing to obtain a mixture as a fermentation substrate, regulating the moisture content, adding 3 kinds of bacteria of phaffia rhodozyma, bacillus subtilis, plant lactobacillus and the like, controlling fermentation condition, and adopting combination of aerobic fermentation and anaerobic fermentation so as to obtain the products namely the microbial cultures. When the method disclosed by the invention is used for producing the microbial cultures through solid state fermentation ofshrimp shells, the possibility that chitin in the shrimp shells is converted into functional chitosan is effectively reduced, the protein content is increased, and the method is suitable for industrial production of feeds.

Description

technical field [0001] The invention relates to the field of feed, in particular to a method for preparing microbial culture by solid-state fermentation of shrimp shells. Background technique [0002] During the processing of shrimp, a large amount of leftovers such as shrimp heads and shrimp shells will be produced, which are collectively referred to as shrimp shells. Shrimp shells are important feed resources in coastal provinces in my country. They are mainly concentrated in Zhejiang, Shandong, Liaoning and other places. Zhejiang is mainly dried. Shandong and Liaoning are mainly sun-dried. Crude protein in shrimp shells is as high as 15-35%, and it is rich in chitin and astaxanthin, which is a resource worth developing and utilizing. Due to the high cost of drying and the smell of drying, the utilization of shrimp shells is limited; the use of microbial fermentation to develop Shrimp shells are valued for their low cost and no pollution. [0003] The existing methods of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/26A23K10/37A23K10/12A23K20/20A23K20/22A23L5/20
CPCA23K10/12A23K10/26A23K10/37A23K20/20A23K20/22A23L5/21A23V2400/169Y02P60/87
Inventor 钱利纯姚磊丁小青
Owner ZHEJIANG UNIV
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