A kind of method utilizing seawater to promote Haematococcus pluvialls to produce natural astaxanthin

Abstract

The invention discloses a method of utilizing seawater to facilitate haematococcus pluvialis to produce natural astaxanthin, and relates to the technical field of artificial cultivation of haematococcus pluvialis. The method comprises the following steps: utilizing seawater to prepare an improved culture medium of haematococcus pluvialis, adding seawater step by step to induce vegetative growth of haematococcus, cell type transformation and accumulation of astaxanthin. The method can be applied to cultivate haematococcus pluvialis at large scale at the coastal region rich in the seawater resource to produce the natural astaxanthin; compared with the conventional cultivation method of using freshwater, the method has the advantages of short production cycle, high astaxanthin content, and low material cost, and can achieve a wide industrialized application prospect.

Description

technical field

[0001] The invention relates to the technical field of artificial cultivation of Haematococcus pluvialis, in particular to a method for promoting the production of natural astaxanthin by using seawater from Haematococcus pluvialls. Background technique

[0002] Astaxanthin is a carotenoid pigment with the chemical name 3,3'-dihydroxy-β-carotene-4,4'-dione, which is naturally present in algae, shrimp, crab and salmon, etc. biological. Natural astaxanthin is a carotenoid with high antioxidant activity. It has 10 times the antioxidant effect of β-carotene and 550 times the inhibitory properties of vitamin E in lipid peroxidation. It cannot be synthesized in animals , but it is a necessary substance for human and animal life activities. Studies have shown that astaxanthin can enhance the immunity of organisms, assist in the recovery of diseases such as cardiovascular disease, immune system, inflammation and neurodegenerative diseases, and at the same time have ...

Examples

Embodiment Construction

[0028] The present invention will be described in further detail below in conjunction with specific examples. The following examples are explanations of the present invention, and the present invention is not limited to the following examples.

[0029] In this example, the wild strain of Haematococcus pluvialis was used as the material, and seawater was added step by step to the culture system of Haematococcus pluvialls, and the time periods of addition were the vegetative growth stage and the cell type conversion stage of Haematococcus pluvialis cells. and algal cell astaxanthin accumulation stage. The seawater is taken from Daya Bay, Shenzhen. The salinity is 3% as measured by the ATAGO S28α salinity meter. The seawater is sterilized by high temperature and high pressure before being added to the culture system, or treated by membrane filtration (the pore size of the filter membrane is 0.22 microns), or by ultraviolet disinfection. Or ozone disinfection treatment, and then a...

technical field

[0001] The invention relates to the technical field of artificial cultivation of Haematococcus pluvialis, in particular to a method for promoting the production of natural astaxanthin by using seawater from Haematococcus pluvialls. Background technique

[0002] Astaxanthin is a carotenoid pigment with the chemical name 3,3'-dihydroxy-β-carotene-4,4'-dione, which is naturally present in algae, shrimp, crab and salmon, etc. biological. Natural astaxanthin is a carotenoid with high antioxidant activity. It has 10 times the antioxidant effect of β-carotene and 550 times the inhibitory properties of vitamin E in lipid peroxidation. It cannot be synthesized in animals , but it is a necessary substance for human and animal life activities. Studies have shown that astaxanthin can enhance the immunity of organisms, assist in the recovery of diseases such as cardiovascular disease, immune system, inflammation and neurodegenerative diseases, and at the same time have ...

Examples

Embodiment Construction

[0028] The present invention will be described in further detail below in conjunction with specific examples. The following examples are explanations of the present invention, and the present invention is not limited to the following examples.

[0029] In this example, the wild strain of Haematococcus pluvialis was used as the material, and seawater was added step by step to the culture system of Haematococcus pluvialls, and the time periods of addition were the vegetative growth stage and the cell type conversion stage of Haematococcus pluvialis cells. and algal cell astaxanthin accumulation stage. The seawater is taken from Daya Bay, Shenzhen. The salinity is 3% as measured by the ATAGO S28α salinity meter. The seawater is sterilized by high temperature and high pressure before being added to the culture system, or treated by membrane filtration (the pore size of the filter membrane is 0.22 microns), or by ultraviolet disinfection. Or ozone disinfection treatment, and then a...