Screening method of haematococcus pluvialis mutant with high yield of astaxanthin

A technology of Haematococcus pluvialis and Haematococcus pluvialis algal liquid, applied in the directions of mutant preparation, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem of laborious and restrictive Haematococcus pluvialis astaxanthin Development and utilization, mutant screening time-consuming and other issues, to achieve the effect of rich content, good development prospects, and high-throughput screening

Inactive Publication Date: 2021-10-08
新疆金正生物科技有限公司
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Problems solved by technology

[0005] Ultraviolet rays have strong biological effects on higher plants, and are effectively and widely used in the mutagenesis breeding of agricultural biological resources. In microalgae, there have been successful examples of using ultraviolet rays to mutagenize algal strains, but the screening of mutants is It is a time-consuming and laborious process; there are few reports on the mutation breeding of Haematococcus pluvialis, and the screening of mutants is time-consuming and laborious, which seriously restricts the development and utilization of Haematococcus pluvialis astaxanthin

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  • Screening method of haematococcus pluvialis mutant with high yield of astaxanthin
  • Screening method of haematococcus pluvialis mutant with high yield of astaxanthin
  • Screening method of haematococcus pluvialis mutant with high yield of astaxanthin

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Embodiment 1

[0032] Such as Figure 1 to Figure 3 As shown, the present invention discloses a method for screening Haematococcus pluvialis mutants with high yield of astaxanthin. The technical solution adopted is to include the following steps:

[0033] In the first step, the algae liquid of Haematococcus pluvialis 6W3 cultured to the logarithmic growth phase was diluted to 10 with distilled water. 5Cells / mL, use a pipette to take 5mL of the dilution and spread it on a petri dish, use a 30W UV lamp to irradiate at 20cm, and the irradiation time is 0min, 2min, 4min, 6min, 8min, 10min, and place it in the dark after irradiation. , carry out cell count respectively on the 3rd day and the 6th day, calculate the lethality rate, the lethality rate calculation formula is:

[0034] Lethal rate (%) = number of dead cells / number of initial cells × 100

[0035] For mutagenesis, in order to achieve effective mutagenesis and avoid excessive radiation dose, which would cause all cells to die, a 30W ul...

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Abstract

The invention discloses a screening method of a haematococcus pluvialis mutant with high yield of astaxanthin, relates to the field of astaxanthin culture and screening, and aims to solve the problems of time consumption and labor consumption in mutant screening in the prior art. The technical scheme is as follows: a mutant algal strain is obtained by performing ultraviolet mutagenesis and screening on haematococcus pluvialis 6W3, the screening method is easy to operate and is low in time consumption in the process, high-throughput screening can be achieved, and the working efficiency of screening is greatly improved. The obtained mutant grows quickly, has high astaxanthin content and has a good astaxanthin development prospect; when the haematococcus pluvialis is cultured, the single algae colony with large volume and dark green color is selected to be cultured, the content of astaxanthin in the single algae colony with large volume and dark green color is rich, the concentration of astaxanthin in the extract liquor is high, and the yield in the subsequent extraction and purification process can be effectively improved.

Description

technical field [0001] The invention relates to the field of astaxanthin cultivation and screening, in particular to a screening method for Haematococcus pluvialis mutants with high astaxanthin production. Background technique [0002] Astaxanthin (3,3'-dihydroxy-β,β'-carotene-4,4'-dione) is a red carotenoid derivative. Studies have shown that astaxanthin has strong dyeing, anti-oxidation and immune-enhancing abilities. Astaxanthin can remove nitrogen dioxide, sulfide, disulfide, etc., and can also reduce lipid peroxidation, effectively inhibiting Lipid peroxidation caused by free radicals; at the same time, it has various physiological effects such as inhibiting tumor occurrence, enhancing immunity, and removing free radicals in the body. It has a good therapeutic effect on skin cancer caused by ultraviolet rays, and has a good therapeutic effect on eye diseases caused by diabetes. It also has a preventive effect. Because the special molecular structure determines its spec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01C12N13/00C12R1/89
CPCC12N15/01C12N13/00
Inventor 梁钧
Owner 新疆金正生物科技有限公司
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