Method for cultivating phaffiafhodozyma using astaxanthin synthesis accelerant

A technology of Phaffia rhodozyma and astaxanthin, which is applied in the directions of fermentation and fungi, can solve the problems of high production cost and low yield of astaxanthin, and achieve the advantages of low raw material cost, high promotion and utilization value, and increased yield. Effect

Inactive Publication Date: 2006-07-26
DALIAN POLYTECHNIC UNIVERSITY
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing research and production practice of using Phaffia rhodozyme microbial fermentation to produce natural

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for cultivating phaffiafhodozyma using astaxanthin synthesis accelerant

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0016] Example 1.

[0017] A. Selection of raw materials: Choose fresh oranges containing Phaffia rhodozyma astaxanthin synthesis precursor, peel and weigh;

[0018] B. Juicing: Squeeze fresh orange to obtain a solid-liquid mixture of juice;

[0019] C. Preparation of accelerant additive liquid: filter the solid-liquid mixture of the squeezed juice, sterilize the filtrate at 115°C for 20 minutes, then filter the precipitate aseptically, determine the reducing sugar content, and store at 4°C for later use;

[0020] D. Liquid seed culture: Activate the slant preserved strain on the 10Bx wort slant medium at 22℃, and cultivate it in YM medium at 22℃ in liquid aeration;

[0021] E. Phaffia rhodozyma fermentation: 8% of the total volume of the YM medium is added to the liquid seeds cultured in step d, and the Phaffia rhodozyma is cultured at 22°C for 24h in liquid ventilation;

[0022] F. Liquid aeration culture: In the cultivated Phaffia rhodozyma, add the promoter additive prepared i...

Example Embodiment

[0025] Example 2.

[0026] Take bananas according to the steps A, B, C, D in Example 1;

[0027] E. Phaffia rhodozyma fermentation: 12% of the total volume of the YM medium is added to the liquid seed cultured in step d, and then the promoter additive prepared in step C is directly added;

[0028] F. Liquid ventilation culture: Add the promoter additive prepared in step C to the cultivated Phaffia rhodozyma at 4% of the total volume of the fermentation broth, continue the fermentation for 96 hours, and collect the yeast cells.

[0029] G. Extract astaxanthin: Determine the content of astaxanthin in yeast cells, then extract astaxanthin according to conventional methods, and then extract bacterial protein.

[0030] The cell yield, astaxanthin yield, and cell astaxanthin content can be increased by 54.4%, 65.5% and 7.2% respectively compared with the control.

Example Embodiment

[0031] Example 3.

[0032] A. Selection of raw materials: Choose fresh spinach containing Phaffia rhodozyma astaxanthin synthesis precursor, remove the roots and yellow leaves, wash and dry, and weigh;

[0033] B. Juicing: Take 700 grams of spinach and add 300 ml of water to squeeze the juice to obtain a solid-liquid mixture of juice;

[0034] C. Preparation of accelerant additive: filter the solid-liquid mixture of the juice, sterilize the filtrate at 110°C for 20 minutes, and filter the precipitate aseptically to obtain light green spinach juice. Determine the reducing sugar content and store at 4°C for later use ;

[0035] D. Liquid seed culture: The slant preserved strain is activated on 10Bx wort slant medium at 25°C, and cultivated in YM medium at 25°C in liquid aeration;

[0036] E. Phaffia rhodozyma fermentation: add 6% of the total volume of the YM medium to the liquid seeds cultivated in step d, and cultivate Phaffia rhodozyma at 25°C for 24 hours in liquid ventilation; ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A method for culturing red-hair yeast with shrimpanin synthesis accelerator. Use shrimpanin synthesis former-rich fruits, vegetables and tea juice such as fresh orange, spinach, tea leaf, etc as materias, and prepare shrimpanin synthesis accelerator by pressing juice or immersion. Add the shrimpanin synthesis accelerator in red-hair yeast medium at different fermention time; ferment for some time; collect yeast cell. Also we can furtherly do cell wall breaking treatment and extrat shrimpanin from the cell with acetone. The red-hair yeast achieved has high shrimpanin content, and the shrimpanin content can be advanced largely just by adding some shrimpanin synthesis accelerator. It has the advantages of investment little, raw materials cost little, and so on. Both the achieved red-hair yeast and the shrimpanin extracted from it can be used as feedingstuff additive for top-grage aquatic products and birds, and can make the culturing products have high commondity value.

Description

technical field [0001] The invention relates to the field of preparation of biologically active substances, in particular to a method for preparing natural astaxanthin by yeast fermentation. Background technique [0002] Astaxanthin is a ketone carotenoid with high economic value, which is bright red. It widely exists in the biological world, especially in aquatic animals such as shrimp, crab, fish and bird feathers. Because astaxanthin has a unique coloring function and strong antioxidant properties, it is far stronger than other carotenoids in biological functions such as anti-cancer, enhancing immunity and anti-photosensitivity, so it is widely used in feed, food, cosmetics and medicine, etc. The field has broad application prospects. According to its source, astaxanthin can be divided into chemical synthesis and natural two categories. At present, chemically synthesized astaxanthin is mainly used as an animal feed additive, such as some ornamental and farmed fish, cru...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/16C12P23/00
Inventor 孙玉梅华艳艳
Owner DALIAN POLYTECHNIC UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap