Method for promoting high yield of astaxanthin from phaffia rhodozyma

Abstract

The invention discloses a method for promoting high yield of astaxanthin from phaffia rhodozyma by TiO2 / Al2O3. The method is characterized by comprising the following steps: a sterile fermentation culture medium added with a nano TiO2 / Al2O3 mother solution is inoculated with a phaffia rhodozyma seed solution for fermentation culture, the nano TiO2 / Al2O3 mother solution is a nano titanium dioxide mixed solution taking a nano aluminum oxide aqueous dispersion as a carrier, and an unbalanced pH value inside and outside phaffia rhodozyma cells is kept during fermentation, so that the stress reaction of the phaffia rhodozyma is stimulated, and a metabolic pathway in the phaffia rhodozyma proceeds towards an astaxanthin production direction. The method has the advantages that the nanometer aluminum oxide aqueous dispersion is used as a carrier, the nanometer titanium dioxide is treated and dissolved into the fermentation culture medium, the unbalanced pH value inside and outside the phaffia rhodozyma cells is kept during fermentation, the stress reaction of the phaffia rhodozyma is stimulated, the metabolic pathway in the phaffia rhodozyma proceeds towards the astaxanthin production direction, and thus, the content of the astaxanthin is increased.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a method for promoting high-yield astaxanthin by Phaffia yeast with TiO2 / Al2O3. Background technique [0002] The chemical name of Astaxanthin is 3,3′-dihydroxy-4,4′-diketone-β,β′-carotene, and its molecular formula is C 40 h 52 o 4 , with a relative molecular mass of 596.86, is a ketocarotenoid and a terpene-like unsaturated compound. Crystalline astaxanthin is pink, with a melting point of 215 / 216°C, insoluble in water, fat-soluble, and easily soluble in most organic solvents such as chloroform, acetone, and benzene. The conjugated double bond chain in the molecular structure of astaxanthin, as well as the unsaturated ketone group and hydroxyl group at the end of the conjugated double bond chain, can attract unpaired electrons from free radicals or provide electrons to free radicals, thereby scavenging free radicals and acting as an antioxidant The structure...

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Problems solved by technology

Although the literature reports that the addition of titanium dioxide can promote the production of astaxanthin by Phaffia yeast, the solid particle size of titanium dioxide mentioned in the literature is only on the micron scale, and since yeast fermentation is usually in aqueous solution, the solubility of micron-scale titanium dioxide is extremely high. Low, almost insoluble in water, so that micron-sized titanium dioxide cannot be efficiently delivered to the yeast cells during fermentation, so the content of astaxanthin in Phaffia yeast cultured by the method reported in this document is low

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