Method for producing astaxanthin by utilizing phaffia rhodozyma

A technology of Phaffia rhodozyma and astaxanthin, which is applied in the field of microorganisms, can solve the problems of harsh culture conditions, high production cost of astaxanthin, slow growth of Haematococcus pluvialis, etc., so as to increase carbon content and promote the accumulation of astaxanthin. hormone, growth-promoting effect

Inactive Publication Date: 2018-05-04
罗世华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The content of astaxanthin in the cells of Haematococcus yushengi is relatively high, which can reach 1% to 3% of the dry weight of the cells. H

Method used

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  • Method for producing astaxanthin by utilizing phaffia rhodozyma

Examples

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Embodiment 1

[0022] A method utilizing Phaffia rhodozyme to produce astaxanthin, comprising the following steps:

[0023] 1) Grinding and pulverizing the shrimp waste to obtain a refining liquid, boiling and cooling the refining liquid, adding compound protease and papain for enzymolysis to obtain an enzymatic hydrolysis liquid; adding straw cellulose dry matter, sulfuric acid to the enzymatic hydrolysis liquid Ammonium and molasses are mixed evenly, and the fermentation substrate is obtained after high-pressure steam sterilization;

[0024] 2) inoculating Phaffia rhodozyme into the activation medium, and culturing it in a shaker to obtain activated Phaffia rhodozyme;

[0025] 3) Inoculate the activated Phaffia rhodozyme into the expansion medium, mix it with the fermentation substrate after cultivating for 50 hours under stirring conditions, feed sterile oxygen for continuous cultivation for 50 hours, feed sterile carbon dioxide, oxygen and Mixed gas composed of ethylene, the volume frac...

Embodiment 2

[0028] A method utilizing Phaffia rhodozyme to produce astaxanthin, comprising the following steps:

[0029] 1) Grinding the shrimp scraps with a colloid mill to 300 mesh, boiling and cooling the slurry, adding compound protease and papain for enzymolysis to obtain an enzymolysis solution; adding straw cellulose dry matter, Ammonium sulfate and molasses are mixed evenly, and the fermentation substrate is obtained after high-pressure steam sterilization;

[0030] 2) inoculating Phaffia rhodozyme into the activation medium, and culturing it in a shaker to obtain activated Phaffia rhodozyme;

[0031] 3) Inoculate the activated Phaffia rhodozyme into the expansion medium, mix it with the fermentation substrate after cultivating under stirring conditions for 45 hours, feed sterile oxygen for continuous cultivation for 45 hours, feed sterile carbon dioxide, oxygen and Mixed gas composed of ethylene, the volume fraction ratio of carbon dioxide, oxygen and ethylene is 2:1:0.03, the v...

Embodiment 3

[0034] A method utilizing Phaffia rhodozyme to produce astaxanthin, comprising the following steps:

[0035] 1) Grinding the shrimp scraps with a colloid mill to 300 meshes, boiling and cooling the slurry, adding a compound protease and papain with a mass ratio of 3:1 for enzymolysis to obtain an enzymolysis solution; Add straw cellulose dry matter, ammonium sulfate and molasses, mix evenly, and obtain a fermentation substrate after high-pressure steam sterilization;

[0036] 2) inoculating Phaffia rhodozyme into the activation medium, and culturing it in a shaker to obtain activated Phaffia rhodozyme;

[0037] 3) Inoculate the activated Phaffia rhodozyme into the expansion medium, mix it with the fermentation substrate after cultivating under stirring conditions for 45 hours, feed sterile oxygen for continuous cultivation for 45 hours, feed sterile carbon dioxide, oxygen and Mixed gas composed of ethylene, the volume fraction ratio of carbon dioxide, oxygen and ethylene is 2...

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Abstract

The invention discloses a method for producing astaxanthin by utilizing phaffia rhodozyma. The method comprises the following steps: 1) grinding and crushing shrimp offal to obtain milled slurry, boiling and cooling the milled slurry, adding compound protease and papain to perform enzymolysis so as to obtain enzymatic hydrolysate; adding straw cellulose dry matters, ammonium sulfate and molasses into the enzymatic hydrolysate, uniformly mixing, and autoclaving to obtain a fermentation matrix; 2) activating phaffia rhodozyma; 3) inoculating the activated phaffia rhodozyma into an expanded medium, culturing under stirred conditions for 45-50 hours, mixing the fermentation matrix, introducing sterile oxygen for continuously culturing for 45-50 hours, introducing mixed gas composed of sterilecarbon dioxide, oxygen and ethylene, wherein the volume ratio of the carbon dioxide to oxygen to ethylene is 2:1:0.03, and the ventilatory capacity is 0.2V/(V.min), and continuously culturing for 45-50 hours so as to obtain the phaffia rhodozyma; and 4) extracting and purifying, thereby obtaining the astaxanthin. The method disclosed by the invention has the characteristics of low production cost,high benefits and the like.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for producing astaxanthin by using Phaffia rhodozyme. Background technique [0002] Astaxanthin is an oxygenated derivative of carotenoids and belongs to the group of keto carotenoids. Astaxanthin has super antioxidant activity, its antioxidant activity is 10 times higher than β-carotene, 500 times higher than vitamin E, and astaxanthin can improve immunity, prevent tumors, cardiovascular diseases and eye diseases And so on. Astaxanthin has broad application prospects in the fields of food, medicine and cosmetics, so the market demand for astaxanthin is very large, and its economic value is very high. [0003] At present, the sources of astaxanthin include biological extraction and chemical synthesis. Chemically synthesized astaxanthin is completely different from natural astaxanthin in terms of structure, function and safety. Therefore, the US FDA has explicit...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/645
CPCC12P23/00Y02P20/54
Inventor 罗世华
Owner 罗世华
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