Method for extracting astaxanthin in haematococcus pluvialis by adopting enzymolysis method and grinding method through organic solvent

A technology of Haematococcus pluvialis and organic solvent is applied in the field of astaxanthin to achieve the effects of shortening time consumption, improving extraction rate and shortening extraction time.

Inactive Publication Date: 2014-06-25
INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem of time-consuming and harsh process conditions in the method of enzymatically hydrolyzing Haematococcus pluvialls to extract astaxanthin, and to further improve the obtained astaxanthin products The purity of cyanidin; the invention provides a method for extracting astaxanthin in Haematococcus pluvialls in conjunction with an enzymatic method and a grinding method in cooperation with an organic solvent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Weigh 15g of Haematococcus pluvialis powder, put it into an agate ball mill jar, and then add 100mL of distilled water;

[0039] (2) Then, add a citric acid solution with a mass concentration of 5% to the ball mill tank to adjust the pH to 4.0;

[0040] (3) Add 0.0075 g of pectinase to the ball mill tank; adjust the temperature of the material in the ball mill tank to 45°C, and grind for 3 hours at a ball mill speed of 150 r / min to obtain an enzymatic solution;

[0041] (4) Centrifuge the enzymolysis solution at 10°C at a centrifugal speed of 5000rmp / min, and then take the bottom solution; remove the water from the bottom solution and dry it to obtain Haematococcus pluvialis enzymatic hydrolysis powder;

[0042] (5) Add 100 mL of ethyl acetate to the enzymatic hydrolysis powder of Haematococcus pluvialis obtained in step (4), and extract at a temperature of 45°C for 60 minutes to obtain a mixture;

[0043] (6) Centrifuge the mixture at 10°C at a centrifugal speed o...

Embodiment 2

[0047] (1) Weigh 20g of Haematococcus pluvialis powder, put it into an agate ball mill jar, and then add 150mL of distilled water;

[0048] (2) Then, add a citric acid solution with a mass concentration of 5% to the ball mill tank to adjust the pH to 5.0;

[0049] (3) Add 0.02 g of pectinase to the ball mill tank; adjust the temperature of the material in the ball mill tank to 50°C, and grind for 4 hours at a ball mill speed of 170 r / min to obtain an enzymatic hydrolysis solution;

[0050](4) Centrifuge the enzymolysis liquid at 10°C at a centrifugal speed of 4000rmp / min, and then take the bottom liquid; remove water from the bottom liquid and dry it to obtain Haematococcus pluvialis enzymatic hydrolysis powder;

[0051] (5) Add 150 mL of ethyl acetate to the enzymatic hydrolysis powder of Haematococcus pluvialis obtained in step (4), and extract at a temperature of 50°C for 75 minutes to obtain a mixture;

[0052] (6) Centrifuge the mixture at 10°C at a centrifugal speed of ...

Embodiment 3

[0056] (1) Weigh 25g of Haematococcus pluvialis powder, put it into an agate ball mill jar, and then add 200mL of distilled water;

[0057] (2) Then, add a citric acid solution with a mass concentration of 5% to the ball mill tank to adjust the pH to 6.0;

[0058] (3) Add 0.05 g of pectinase to the ball mill tank; adjust the temperature of the material in the ball mill tank to 60°C, and grind for 5 hours at a ball mill speed of 200 r / min to obtain an enzymatic hydrolysis solution;

[0059] (4) Centrifuge the enzymolysis solution at 10°C at a centrifugal speed of 6000rmp / min, and then take the bottom solution; remove water from the bottom solution and dry it to obtain Haematococcus pluvialis enzymatic hydrolysis powder;

[0060] (5) Add 200 mL of ethyl acetate to the enzymatic hydrolysis powder of Haematococcus pluvialis obtained in step (4), and extract at a temperature of 55°C for 90 minutes to obtain a mixture;

[0061] (6) Centrifuge the mixture at 10°C at a centrifugal sp...

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Abstract

The invention relates to a method for extracting astaxanthin in haematococcus pluvialis by adopting an enzymolysis method and a grinding method through an organic solvent, belonging to the field of algal pigment extraction technology. The enzymolysis time for extracting astaxanthin by adopting the existing enzymolysis method is generally more than 15 hours, and the whole extraction process only needs 3-6 hours by adopting the method through assisting the extraction of the organic solvent while grinding in the enzymolysis process, so that the time can be shortened by 57-78%, and the time cost can be greatly saved. The method comprises the steps of carrying out enzymolysis by adopting pectinase, wherein the enzymolysis pH value is 4.0-6.0; firstly drying the enzymolysis liquid, and then extracting with ethyl acetate. The extraction rate can achieve more than 97% by matching pectinase enzymolysis with specific grinding conditions as well as specific organic solvent extraction, and the purity of the obtained astaxanthin product can achieve more than 16.9%, namely that the extraction rate and purity of the astaxanthin can be further improved under the condition of greatly shortening the extraction time.

Description

technical field [0001] The invention relates to a method for extracting astaxanthin from Haematococcus pluvialis in cooperation with an enzyme method and a grinding method, and belongs to the technical field of algae pigment extraction. Background technique [0002] Haematococcus pluvialis ( Haematoccoccus pluvialis ) is a widely distributed unicellular green algae, which can form chlamydospores and accumulate a variety of carotenoids with physiological functions in large quantities under stress conditions: astaxanthin, α-carotene, β-carotene, lutein 80% of which are astaxanthin and its esters. Astaxanthin is a natural carotenoid, which is fat-soluble and pink in color. It contains unsaturated compounds in terpenes. It is a strong antioxidant that can quench superoxide radicals and singlet oxygen ions. Clinical and animal experiments have proved that astaxanthin has various biological functions such as increasing immune function, inhibiting tumor occurrence, preventing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
Inventor 赵晓燕陈军王宪昌张奇志邓鹏毕玉平王兴军
Owner INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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