Method for producing astaxanthin by haematococcus pluvialis

Abstract

The invention discloses a method for producing astaxanthin by haematococcus pluvialis. The method comprises the steps of: firstly, naturally culturing the haematococcus pluvialis in water of a culture pool for 6-8 days so that the haematococcus pluvialis proliferates, then adding potassium dihydrogen phosphate and sodium nitrate to the pool water, and covering the pool with a red thin film, so that algae cells of the haematococcus pluvialis rapidly grow to reach conversion state under the irradiation of 630nm-760nm light waves; transferring the algae cells at the conversion state to pool water with potassium dihydrogen phosphate, the final concentration of which is 0.02-0.05g / L, covering the pool with a blue thin film, so that the algae cells of haematococcus pluvialis are cultivated to mature under the irradiation of 430nm-490nm light waves, and thus obtaining haematococcus pluvialis, the algae cells of which are rich in astaxanthin, wherein potassium dihydrogen phosphate, sodium nitrate and red lights can enable the algae cells to rapidly grow and reach conversion state in shorter time, and the potassium dihydrogen phosphate and blue lights can enable the algae cells to rapidly grow, mature and accumulate astaxanthin. The method has the advantages of little investment, cultivation simpleness and high economic benefits.

Description

technical field [0001] The invention relates to a method for producing astaxanthin, in particular to a method for producing astaxanthin from Haematococcus pluvialis. Background technique [0002] Haematococcus pluvialis ( Haematococcus pluvialis ) is a single-celled green algae, which is currently known as the organism with the highest astaxanthin content. Haematococcus pluvialis can accumulate astaxanthin as high as 1.5% to 3.0% of its dry weight, so it is regarded as a natural A "concentrate" of astaxanthin. At present, there are many ways to produce astaxanthin by using Haematococcus pluvialis, such as the invention patent of authorized announcement number CN1181184, which uses complex culture agents to expand the growth and convert into astaxanthin in a bioreactor to reach astaxanthin. Accumulation purpose; the invention patent with the authorized announcement number CN1004536325 uses a complete set of equipment to control the growth of Haematococcus pluvialis and conv...

AI Technical Summary

Problems solved by technology

At present, there are many ways to produce astaxanthin by using Haematococcus pluvialis, such as the invention patent of authorized announcement number CN1181184, which uses complex culture agents to expand the growth and convert into astaxanthin in a bioreactor to reach astaxanthin. Accumulation purpose; the invention patent with the authorized announcement number CN1004536325 uses a complete set of equipment to control the growth of Haematococcus pluvialis and convert it into astaxanthin with LED light source in the culture medium with appropriate nitrogen and phosphorus content; these two kinds of inputs High cost, complex growth regulation, low economic benefit

The authorized announcement number is CN101586140 invention patent, which discloses that in the nitrogen and phosphorus content culture solution, by controlling different pH values, culture temperature, light intensity, light time, and adding mineral salts, several stages of culture can be achieved to achieve growth and transformation, and then add H 2 o 2 , FeCl 2 Cultivate and accumulate astaxanthin, the input cost is high, the cultivation is complicated, and the economic benefit is low