Method for producing astaxanthin by inducing Haematococcus pluvialis

A technology of Haematococcus pluvialis and astaxanthin, which is applied in the field of microorganism application, can solve problems such as inability to meet high-efficiency utilization, and achieve the effects of increasing yield, simple and easy method, and accelerating accumulation process.

Active Publication Date: 2014-12-24
BIOALGO CY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for better control over how much CO2 enters cells during their growing processes compared to traditional methods like bacteria cultures. By introducing enough oxygen through an artificial atmosphere called Carbon Dioxide (CO2) instead of relying solely upon photosynthesis alone, this results in faster formation of green-house gases such as CH4 and HC1. Additionally, it provides strong mixing between different types of atmospheres when combining them together at once, resulting in even greater distribution within cellular structures. Overall, these technical improvements lead to improved yield rates and reduced costs associated with natural pigmentation from marine organisms.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding ways to quickly make naturally occurring astaxytocopherols (a type called 3α-, 4β-, or 7E)-derived compounds with high bioactivity without losing their ability to bind tightly against certain molecules like proteins involved in various physiology processes including metabolism, tissue regenerations, cancer development, etc., making them useful drugs for use in medicine purposes.

Method used

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  • Method for producing astaxanthin by inducing Haematococcus pluvialis

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Embodiment 1

[0029] Step 1. Massive propagation of algae species: inoculate Haematococcus pluvialis into the propagation medium, at a temperature of 22°C and a continuous light intensity of 20 μmol·m -2 ·s -1 And at the same time continue to pass into the breeding medium through filtering and mixed with the air condition of 1% carbon dioxide to propagate, and its feed rate is to feed 0.2L / min in every 1L of breeding medium, until Reach the end of the logarithmic growth phase of Haematococcus pluvialis, and obtain a large number of algae species;

[0030] Step 2. Production of astaxanthin: Inoculate Haematococcus pluvialls into the production medium, and produce astaxanthin at a temperature of 22°C, wherein the light on Haematococcus pluvialls is continuously maintained during the cultivation process, and Ensure that the light intensity reaching the cell surface of Haematococcus pluvialis is 20 μmol m -2 ·s -1 Adding ethanol to the nutrient medium at the same time, wherein the ethanol ad...

Embodiment 2

[0033] Step 1. Massive propagation of algae species: inoculate Haematococcus pluvialis into the propagation medium, at a temperature of 24°C and a continuous light intensity of 60 μmol·m -2 ·s -1 And at the same time continue to pass into the breeding medium through filtering and mixed with the air condition of 1% carbon dioxide to propagate, and its feed rate is to feed 0.2L / min in every 1L of breeding medium, until Reach the end of the logarithmic growth phase of Haematococcus pluvialis, and obtain a large number of algae species;

[0034] Step 2, producing astaxanthin: Inoculate Haematococcus pluvialls into the production medium, and produce astaxanthin at a temperature of 24°C, wherein the light on Haematococcus pluvialls is continuously maintained during the cultivation process, and Ensure that the light intensity reaching the cell surface of Haematococcus pluvialis is 60 μmol m -2 ·s -1 Adding ethanol and sea crystals simultaneously in the production medium, wherein t...

Embodiment 3

[0037] Step 1. Massive propagation of algae species: inoculate Haematococcus pluvialis into the propagation medium, at a temperature of 26°C and a continuous light intensity of 100 μmol·m -2 ·s -1And at the same time continue to pass into the breeding medium through filtering and mixed with the air condition of 2% carbon dioxide to propagate, and its feed rate is to feed 0.3L / min in every 1L of breeding medium, until Reach the end of the logarithmic growth phase of Haematococcus pluvialis, and obtain a large number of algae species;

[0038] Step 2. Production of astaxanthin: inoculate Haematococcus pluvialls into the production medium, and produce astaxanthin at a temperature of 26°C, wherein the light on Haematococcus pluvialls is continuously maintained during the cultivation process, and Ensure that the light intensity reaching the cell surface of Haematococcus pluvialis is 100 μmol m -2 ·s -1 Add ethanol and sea crystal simultaneously in the production medium, wherein ...

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Abstract

The invention discloses a method for producing astaxanthin by inducing Haematococcus pluvialis, which is characterized by comprising the following steps: in the vegetative growth phase, inoculating Haematococcus pluvialis in a nutrient culture medium, and culturing; continuously keeping irradiation on the Haematococcus pluvialis and ensuring that the intensity of illumination arriving at the Haematococcus pluvialis cell surface is 20-200 mu mol.m<-2>.s<-1>; in the induction stage, adding ethanol and seawater crystal into the production culture medium, wherein the ethanol added into the production culture medium accounts for 0.5-3 vol%, and the seawater crystal added into the production culture medium accounts for 0.5-1 wt%; and continuously introducing filtered air mixed with 1-3 vol% carbon dioxide into the production culture medium, wherein 0.2-0.4L/minute of the air is introduced to every 1L of production culture medium. The method can induce the Haematococcus pluvialis to quickly accumulate the astaxanthin, is simple and has the advantages of high yield and favorable effect.

Description

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Claims

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Application Information

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Owner BIOALGO CY CO LTD
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