Method for achieving high yield of astaxanthin through haematococcus pluvialis

Abstract

The invention relates to a method for achieving high yield of astaxanthin through haematococcus pluvialis. The method comprises the steps that 1, stable-period cultivation is conducted; 2, amplifying cultivation of algal cells is conducted till the cell density does not rise any more; 3, stress cultivation is conducted and used for accumulation of haematococcus pluvialis astaxanthin, the cultivation temperature ranges from 18 DEG C to 33 DEG C, illumination adopts unblocked sunlight, and the sunlight intensity is 10,000 Lux or above. According to the method for achieving the high yield of the astaxanthin, a BBM cultivation medium which has the same salinity and is lack of nitrogen, phosphate and sulfur is used under the high light condition for stress cultivation, the yield of the astaxanthin can be increased to the maximum extent, a double-layer filter cloth is used for filtration, insect attacks introduced in the cultivation process can be removed, and harvesting of haematococcus pluvialis cells at the last period of cultivation is convenient.

Description

technical field [0001] The invention belongs to the field of producing astaxanthin by haematococcus, and relates to a method for high-yielding astaxanthin by haematococcus pluvialls. Background technique [0002] Astaxanthin, a red carotenoid found in the shell of langoustines, oysters and salmon, the chemical name is 3,3′-dihydroxy-4,4′-diketoyl beta-carotene, the crystal is Maroon. Astaxanthin is a carotenoid with antioxidant activity. Compared with carotene and vitamin E, astaxanthin has stronger biological activity and is a safe substance for human body. The antioxidant activity of astaxanthin is about 10 times higher than that of β-carotene, and about 500 times higher than that of vitamin E. Astaxanthin has been considered as "super vitamin E", and it has a coloring function. It is used in aquatic products and poultry farming, Food, cosmetics, medicine and other fields have broad application prospects. At present, Haematococcus pluvialis is the biological source with...

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Problems solved by technology

[0005] 1. Patent (200910099708.6) "A Simple Method for Cultivating Haematococcus Pluvialls to Produce Astaxanthin". This method is relatively simple through a three-step cultivation method of Erlenmeyer flasks, plastic barrels or nylon bags, and cement pools, but each step All need to control various physical and chemical environmental parameters, which is relatively cumbersome

[0006] 2. Patent (02138827.X) "A Method for Cultivating Haematococcus Pluvialls to Produce Astaxanthin". Although this method is relatively simple, it only uses CO 2 Inducing cells to accumulate astaxanthin is relatively slow, and the harvesting method is more troublesome

However, this method needs to be carried out in a closed photobioreactor and needs to be cultivated in natural water bodies, which is difficult to implement, and the cost of producing astaxanthin is high and the output is small.

[0009] 5. The article "Discussion on Haematococcus Pluvialls Cultivation and Astaxanthin Accumulation Conditions", the article found that nitrogen-deficient medium is the most beneficial for the accumulation of astaxanthin in Haematococcus pluvialis cells, but it is limited to laboratory research

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