Method for producing astaxanthin by haematococcus pluvialis induced by methyl jasmonate

Abstract

The invention provides a method for producing astaxanthin by haematococcus pluvialis induced by methyl jasmonate, which comprises the following steps: (1) culturing methyl jasmonate cells to exponential phase so as to obtain cultured microalgal liquid for induction; and (2) accumulation of astaxanthin: adding methyl jasmonate concentrate the mass concentration of which is 95 percent into cultured microalgal liquid until the final concentration of the methyl jasmonate is 4.95 to 5.05 mg / L; then culturing the microalgal liquid added with methyl jasmonate until the haematococcus pluvialis cells are completely red under an optical microscope at a temperature of 25 to 28 DEG C and an optical intensity of 4500 to 5000lx and under the conditions of continuous illumination of fluorescent lamps and nutritive salt starved compound stresses so as to finish the accumulation of astaxanthin, wherein at the moment of finishing the accumulation of astaxanthin, the maximum output of the astaxanthin is reached. The invention is simple, easy to operate and low in cost, can greatly shorten the time that the haematococcus pluvialis cells are completely red, and significantly increase the output of the astaxanthin, thereby greatly improving the efficiency of producing astaxanthin by haematococcus pluvialis.

Description

technical field

[0001] The invention provides a method for using methyl jasmonate to induce Haematococcus pluvialls to produce astaxanthin, and belongs to the field of biotechnology. Background technique

[0002] Haematococcus pluvialis is recognized as the organism with the highest natural astaxanthin content in nature at present, and its accumulation can be as high as 4.0% of the dry cell weight, which is 1000-4000 times that of salmon meat and far higher Astaxanthin (0.15%-0.4%) produced by fermentation of Phaffia rhodoxyma. Astaxanthin molecules not only have long conjugated double bonds like other carotenoids, but also have unsaturated ketone and hydroxyl groups at the end of the conjugated double bonds, and the hydroxyl and ketone groups form α-hydroxy ketones. This structure endows astaxanthin with more prominent physiological functions than general carotenoids, which are mainly reflected in the following two aspects: First, astaxanthin, like other carotenoids, has a...