Method for alcohol fermentation by immobilization of yeast cell

Abstract

The invention discloses an alcohol ferment production method of fixed yeast cell, which comprises the following steps: adding 10 mL 10 percent yeast suspension liquid in the 90 mL 9 percent polyvinyl alcohol composite gel solution; stirring evenly; pouring the solution in the culture dish to pave; disposing 24-48 h at -20--30 deg.c; cutting the mould into block shape with 12mm*10mm*12mm appearance; breeding in the 12 degreeBe' wort culture medium within 40 percent wort and 60 percent crop mash for 20-48 h at 20-32 deg.c with 100 r per min; adding the fixed yeast in the 16 Bx crop sugar liquid (pH 4.5-4.8) according to 4-8 percent to proceed interval or half-continuous alcohol ferment. The inveiton displays stable operation and high mechanic strength without poisonous and side effect, which simplifies the preparation and reduces the cost.

Description

Technical field:

[0001] The invention relates to a production method for alcohol fermentation using immobilized yeast cells. Specifically, it is a production method of alcoholic fermentation with polyvinyl alcohol composite gel as the carrier of immobilized yeast cells. Background technique:

[0002] Immobilized cells are a high-tech that confines biologically active cells in a certain space and still maintains their unique biological characteristics (such as reproduction and growth metabolism), and can be reused.

[0003] The yeast cells are fixed in the carrier, and after multiplication and culture, they are used in alcohol fermentation production. Compared with the traditional free cell alcohol fermentation, it has the characteristics of fast growth and reproduction of yeast cells, large number of cells, strong anti-bacteria ability, high fermentation efficiency, etc., and can greatly speed up the fermentation speed, shorten the fermentation cycle, effectively improve th...

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