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Method for preparing yeast cell wall beta-1,3-dextran

A technology of yeast cell wall and dextran, which is applied in fermentation and other directions, can solve the problems of inconvenient industrial scale use of technology, few industrial scale research, and inconvenient source of raw materials, so as to save equipment investment, large amount of raw materials, and reduce production costs Effect

Inactive Publication Date: 2008-06-25
天津市工业微生物研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] There are following deficiencies in the above method: 1, raw material problem: some experiments in the literature report adopt fresh yeast as raw material, and such raw material source is inconvenient, and cost is higher
2. Process issues: Most of the literature reports are small-scale experiments in the laboratory, and there are few reports on industrial-scale research. Some processes are not suitable for industrial-scale use
3. Post-processing problem: After a large amount of yeast is processed, it forms a mixed state of soluble substances and dextran. If it is separated by centrifugation, large-scale centrifugal equipment is required, and a large amount of electric energy is consumed
4. Drying problem: Existing methods mostly use organic solvents for dehydration in the post-processing process, and industrial production has the danger of inflammability and explosion

Method used

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  • Method for preparing yeast cell wall beta-1,3-dextran

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Take 500g of beer dry yeast and add 4.5 liters of water, mix evenly, remove impurities such as wheat husk through an 80-mesh sieve, then add NaOH to make the final concentration of NaOH 4%, heat to 100°C, stir at 350 rpm for 3 hours, and let stand Overnight, discard the supernatant the next day, pour the same amount of water as the discarded supernatant, add NaOH to make the final concentration 4%, heat to 100°C, stir at 350 rpm for 3 hours, let stand and discard Remove the supernatant, pour in clean water, repeatedly wash the obtained sediment, and spray dry to obtain dextran.

Embodiment 2

[0029] Take 500g of beer dry yeast and add 4.5 liters of water. After mixing evenly, remove impurities such as wheat husks through a 60-mesh sieve, then add NaOH to make the final concentration of NaOH 4.5%, heat to 110°C, and stir at 300 rpm for 3.5h. Leave it overnight, discard the supernatant the next day, pour in the same amount of water as the discarded supernatant, add NaOH to make the final concentration 5%, heat to 110°C, stir at 350 rpm for 3 hours, and let it stand The supernatant was discarded, poured into clean water, the sediment obtained was repeatedly washed, and spray-dried to obtain dextran.

Embodiment 3

[0031] Take 1Kg of beer dry yeast and add 4.5 liters of water, mix well, remove impurities such as wheat husk through an 80-mesh sieve, then add NaOH to adjust the pH value to 8, add alkaline protease, and react at 55°C for 2 hours; add 5L of water, then add NaOH made the suspension concentration 1%, heated to 100°C, stirred and reacted at 300 rpm for 3 hours, left to stand and discarded the supernatant, poured into clean water, repeatedly washed the sediment obtained, and spray-dried to obtain dextran.

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Abstract

The invention relates to a preparation method for yeast cytoderm beta-1,3-glucans, comprising the following steps: firstly, impurity treatment, namely yeast dry powder is dispersed into water and impurities in the yeast dry powder are filtered; secondly, alkali treatment, namely alkalis are added into filtered yeast suspension and final concentration of alkalis in the suspension is still 0.5 to 7 percent; thirdly, cleaning, namely supernatants are absorbed by means of siphonage until that the supernatants are clear without impurities and PH value is 7 to 8; fourthly, sponging drying, and beta-1,3-glucan solid powder is obtained finally. The invention has the advantages that: firstly, commercial process cost is reduced; secondly, because operating procedures and devices used are simple, the method is easy to realize commercial process; thirdly, protein removal rate in raw materials is high, and yield and purity of the product beta-1,3-glucans are high.

Description

Technical field: [0001] The invention relates to a method for extracting yeast cell wall β-1,3-glucan from waste beer yeast. Background technique: [0002] Sugars are one of the main components of living organisms, and polysaccharides are another polymer compound besides proteins and nucleic acids. Today, with the vigorous development of research on biologically active substances, polysaccharides have become highly valued due to their unique biological functions. One of the functional factors. [0003] The important source of fungal glucan is Saccharomy cescerviseae. S.cerevisiae is one of the oldest microorganisms used by humans. It is safe and non-toxic, and has a wide range of applications in the food and pharmaceutical industries. Yeast cells can use cheap medium, reproduce quickly, and have high biomass yield, which is suitable for large-scale industrial production. Yeast cells are surrounded by cell walls. Generally, yeast cell walls account for 20 to 25% of the dry ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/08C08B37/02
Inventor 姜文侠王海燕张英筠魏呐储兆坤
Owner 天津市工业微生物研究所有限公司
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