Flow cytometry detection method for yeast concentration

A technology of flow cytometry and detection method, applied in measurement devices, individual particle analysis, scientific instruments, etc., can solve the problems of poor repeatability, large operation error, less application of yeast fermentation monitoring, and achieve high accuracy and easy operation. Fast, high-volume results

Inactive Publication Date: 2013-08-07
广州南沙珠江啤酒有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, hemocytometer method is generally used to determine the concentration of yeast, but this method has large operational errors, poor repeatability, and time-consuming
Flow cytometry is a single cell-based detection technology, which has

Method used

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  • Flow cytometry detection method for yeast concentration
  • Flow cytometry detection method for yeast concentration
  • Flow cytometry detection method for yeast concentration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Determination of brewer's yeast concentration during beer fermentation using a flow cytometry assay comprising the following steps:

[0026] 1) Determination of the sample loading speed of the flow cytometer: the flow cytometer used is produced by BD Company, the model is FACSCalibur; randomly add 1 / 2-3 / 4 tubes of distilled water to the flow cytometer tube, weigh the tube The total weight of water and water is recorded as the initial weight; then the sample is loaded at a slow speed, and the sample is stopped immediately after 10 minutes of sample loading, and the sample loading tube is removed, and the total weight of the weighing tube and water is recorded as the final weight; calculated according to the sample loading speed Formula: sample loading speed (ml / min) = (initial weight - final weight) ÷ water density (1g / ml) ÷ sample loading time (10min), calculate the sample loading speed of the flow cytometer in the slow state, repeat the measurement 5 times, the average...

Embodiment 2

[0030] Determination of brewer's yeast concentration during beer fermentation using a flow cytometry assay comprising the following steps:

[0031] 1) Determination of the sample loading speed of the flow cytometer: the flow cytometer used is produced by BD Company, the model is FACSCalibur; randomly add 1 / 2-3 / 4 tubes of distilled water to the flow cytometer tube, weigh the tube The total weight of water and water is recorded as the initial weight; then the sample is loaded at a slow speed, and the sample is stopped immediately after 10 minutes of sample loading, and the sample loading tube is removed, and the total weight of the weighing tube and water is recorded as the final weight; calculated according to the sample loading speed Formula: sample loading speed (ml / min) = (initial weight - final weight) ÷ water density (1g / ml) ÷ sample loading time (10min), calculate the sample loading speed of the flow cytometer in the slow state, repeat the measurement 5 times, the average...

Embodiment 3

[0035] Determination of brewer's yeast concentration during beer fermentation using a flow cytometry assay comprising the following steps:

[0036] 1) Determination of the sample loading speed of the flow cytometer: the flow cytometer used is produced by BD Company, the model is FACSCalibur; randomly add 1 / 2-3 / 4 tubes of distilled water to the flow cytometer tube, weigh the tube The total weight of water and water is recorded as the initial weight; then the sample is loaded at a slow speed, and the sample is stopped immediately after 10 minutes of sample loading, and the sample loading tube is removed, and the total weight of the weighing tube and water is recorded as the final weight; calculated according to the sample loading speed Formula: sample loading speed (ml / min) = (initial weight - final weight) ÷ water density (1g / ml) ÷ sample loading time (10min), calculate the sample loading speed of the flow cytometer in the slow state, repeat the measurement 5 times, the average...

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Abstract

The present invention discloses a flow cytometry detection method for yeast concentrations, and the detection method comprises the steps of: 1) determination of a sample loading rate for a flow cytometry; 2) preparation of a yeast suspension; and 3) determination of the yeast concentration by using the flow cytometry which comprises: delineating a yeast range on an FSC-H/SSC-H scattergram; and loading each sample for 1min, according to a delineated yeast scope, analyzing the proportion of the yeast number accounting for the total particle number, calculating the number of yeasts read in 1min, and calculating the yeast concentration according to a yeast concentration calculation formula. The method of the invention has a large detection amount, high accuracy, good repeatability, and simple and rapid operations, providing a method and a basis for yeast concentration determination.

Description

technical field [0001] The invention relates to a method for detecting yeast concentration, which belongs to the technical field of bioengineering, in particular to a method for detecting yeast concentration by flow cytometry. Background technique [0002] Wine making (such as wine, beer, etc.) goes through a fermentation process. There are many factors that affect the fermentation process, such as the composition and concentration of the fermentation broth, dissolved oxygen content, temperature, pH value, stirring speed, type, activity and concentration of yeast, and so on. Among the above factors, the yeast concentration is the most important process parameter, because under certain conditions, the fermentation yield is closely related to the yeast concentration. [0003] The presence of yeast changes the electrical properties of the fermentation broth, which can be adequately described by permittivity and conductivity in the radio frequency range. The experiment confirm...

Claims

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Application Information

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IPC IPC(8): G01N15/14
Inventor 张秀霞熊丹李惠萍蔡红远张宇锋
Owner 广州南沙珠江啤酒有限公司
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