Preparation method of magnetic biological microcapsule for processing organic matters and ammonia nitrogen in water

A technology for organic matter and water treatment, applied in the direction of fixing on/in organic carriers, fixing on or in inorganic carriers, etc., can solve the problems of microbial instability and large environmental impact, and achieve good biocompatibility , uniform particle size, good adsorption and permeability effects

Active Publication Date: 2015-09-16
上海艾耐基科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for preparing magnetic biological microcapsules for treating organic matter and ammonia nitrogen in water, aiming at the problem that microorganisms are unstable and greatly affected by the environment in the existing microcapsule biological treatment technology

Method used

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  • Preparation method of magnetic biological microcapsule for processing organic matters and ammonia nitrogen in water

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Embodiment 1

[0018] Embodiment 1: the preparation method of a kind of magnetic biological microcapsule of the present invention is as follows:

[0019] 2% calcium chloride solution and 7.0% normal saline were prepared in advance, both in mass concentration.

[0020] (1) Add 1ml of Candida seed solution into 150ml of YPD medium, inoculate the concentration of Candida seed solution, preferably above 1.5 in terms of OD value, and place it in a constant temperature air bath incubator for cultivation. 180rpm, 26°C, after 5.5h, take 20ml of the strain solution in the logarithmic phase, the OD value is 1.3, dry and freeze at low temperature, 3500rpm, 5.5min. Pour off the supernatant and wash 3 times with 7% saline. Suspend the extracted strains with 10ml of normal saline, and oscillate on a vortex shaker to mix evenly to make a suspension of yeast strains, with an OD value of about 1.6.

[0021] (2) Weigh 0.2g sodium alginate and 0.02g Fe 3 o 4 After mixing the powder, add about 9ml of deioni...

Embodiment 2

[0024] Embodiment 2: the preparation method of a kind of magnetic biological microcapsule of the present invention:

[0025] Pre-prepared 2.1% calcium chloride solution, 6% saline, both in mass concentration.

[0026] (1) Add 1ml of Candida seed solution into 140ml of YPD medium, inoculate the concentration of Candida seed solution, preferably above 1.5 in terms of OD value, and place it in a constant temperature air bath incubator for cultivation. 200rpm, 25°C, after 5h, take 20ml of the strain solution in the logarithmic phase, the OD value is 1.2, dry and freeze at low temperature, 4000rpm, 5min. Pour off the supernatant and wash 3.5 times with 6% saline. Suspend the extracted strains with 10ml of normal saline, and oscillate on a vortex shaker to mix evenly to make a suspension of yeast strains with an OD value of about 1.5.

[0027] (2) Weigh 0.19g sodium alginate and 0.018g Fe 3 o 4 After mixing the powder, add about 9ml of deionized water, heat until the sodium algi...

Embodiment 3

[0029] Embodiment 3: the preparation method of a kind of magnetic biological microcapsule of the present invention:

[0030] Pre-prepared 1.9% calcium chloride solution, 8% saline, both in mass concentration.

[0031] (1) Add 1ml of Candida seed solution into 160ml of YPD medium, inoculate the concentration of Candida seed solution, preferably above 1.5 in terms of OD value, and place it in a constant temperature air bath incubator for cultivation. 150rpm, 28°C, after 6h, take 20ml of the strain solution in the logarithmic phase, the OD value is 1.4, dry and freeze at low temperature, 3000rpm, 6min. Pour off the supernatant and wash 4 times with 8% saline. Suspend the extracted strains with 10ml of normal saline, and oscillate on a vortex shaker to mix evenly to make a suspension of yeast strains, with an OD value of about 1.8.

[0032] (2) Weigh 0.21g sodium alginate and 0.022g Fe 3 o 4 After mixing the powder, add about 9ml of deionized water, heat until the sodium algin...

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Abstract

The invention discloses a preparation method of a magnetic biological microcapsule for processing organic matters and ammonia nitrogen in water. The method comprises the following steps: 1, adding Candida yeast living cells into a disinfected YPD culture medium, culturing at 25-28DEG C under 150-200rpm for 5-6h, taking a bacterial strain liquid obtained after a logarithmic phase, carrying out low temperature drying refrigeration, centrifuging, washing, and carrying out suspension extraction by using normal saline to obtain a bacterial strain Candida yeast suspension with the OD value of 1.5-1.8; 2, mixing sodium alginate and Fe3O4 powder, adding the obtained mixture to deionized water according to the addition amount of sodium alginate of 19-21g / L and the addition amount of the Fe3O4 powder of 1.8-2.2g / L, heating until sodium alginate is completely dissolved, and cooling; and 3, uniformly mixing the yeast suspension with the obtained sodium alginate-iron powder solution according to a volume ratio of 1:9-1:11, adding the obtained solution mixture into a calcium chloride solution in a dropwise manner, curing for 30-40min, and filtering to obtain the microcapsule. The magnetic biological microcapsule prepared in the invention can efficiently degrade the organic matters and ammonia nitrogen in water, can maintain the vitality of thalli, separates the thalli from extraneous adverse environment, can be uniformly distributed in water and separated from water, and is stable in the storage and use process.

Description

technical field [0001] The invention belongs to the fields of environmental water treatment and bioengineering, and relates to a preparation method of magnetic biological microcapsules for treating organic matter and ammonia nitrogen in water. Background technique [0002] my country's water resources are scarce and pollution is becoming more and more serious. In the case of fresh water shortage, water plants have to use slightly polluted raw water as a source of drinking water. The main pollutants of slightly polluted raw water are organic matter and ammonia nitrogen. Among the many treatment technologies for slightly polluted water bodies, biotechnology is considered to be one of the most promising technologies due to its high treatment efficiency and no secondary pollution. Among them, the yeast water treatment technology developed in the 1970s has been widely used in the treatment of heavy metals, petroleum, food, and printing and dyeing wastewater. [0003] In drinking...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10C12N11/04
Inventor 刘新续史俊王琳蒋立群
Owner 上海艾耐基科技股份有限公司
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