Novel process for high-effective extraction of carotenoid in Blakeslea trispora

A technology of carotene and boulardii, which is applied in the purification/separation of hydrocarbons, hydrocarbons, organic chemistry, etc. It can solve the problems of complex carotenoid crystal process, difficulty in guaranteeing crystal purity and quality, and unfavorable efficiency for industrial production. , to achieve the effect of being beneficial to industrial production, less organic solvent, and simple operation

Inactive Publication Date: 2013-04-03
INNOBIO CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The preparation of high-purity carotenoid crystals from fermentation products currently has problems such as complex process, large amount of solve

Method used

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  • Novel process for high-effective extraction of carotenoid in Blakeslea trispora
  • Novel process for high-effective extraction of carotenoid in Blakeslea trispora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] ①Take 40g of dry lycopene mycelium, in which the total carotenoid content is 5.1%. The lycopene and β-carotene account for 80% and 20% respectively by HPLC, and 200ml of ethyl acetate is added to the mycelium. , grind with a ball mill for 30min at 25°C, extract the carotenoids in the mycelium while breaking the cell wall, filter, and collect the filtrate;

[0053] 2. Add 200 ml of ethyl acetate to the filter residue of step 1, grind at 25°C for 30min with a ball mill, filter, and collect the filtrate; add 200ml of ethyl acetate to the filter residue, grind at 25°C for 30min with a ball mill, filter, and collect the filtrate;

[0054] 3. The filtrate obtained in the combined steps 1. and 2. is concentrated under vacuum to obtain 39.4 g of oleoresin with a total carotenoid content of 5.12% at 50°C; the extraction rate is 98.9%;

[0055] 4. Add 400 ml of ethanol to the oleoresin in step 3, stir and wash at 50°C for 30min, filter to remove the solution, and dry at 40°C for ...

Embodiment 2

[0058] ①Take 40g dry lycopene mycelium, in which the total carotenoid content is 5.3%, and lycopene and β-carotene account for 79% and 21% respectively by HPLC detection, add 400ml dichloromethane to the mycelium , Homogenize under high pressure for 30min at 25℃, extract the carotenoids in the mycelium while breaking the cell wall, filter, and collect the filtrate;

[0059] ②Add 200ml of dichloromethane to the filter residue of step ①, homogenize under high pressure for 30min at room temperature, filter,

[0060] Collect the filtrate; add 100 ml of dichloromethane to the filter residue, homogenize under high pressure for 30 min at 25°C, filter, and collect the filtrate;

[0061] 3. the filtrate obtained in the combined steps 1. and 2. is concentrated in a vacuum at 50° C. to obtain 28.6 g of oleoresin with a total carotenoid content of 6.79%, and the extraction rate is 91.6%;

[0062] 4. Add 400 ml of ethanol to the oleoresin in step 3, stir and wash at 50°C for 30min, filter...

Embodiment 3

[0065] ①Take 40g of dry β-carotene mycelium, in which the total carotenoid content is 6.5%. The β-carotene and lycopene account for 98% and 2% respectively by HPLC, and 400ml of n-hexane is added to the mycelium. , sheared at 25°C for 30min, the carotenoids in the mycelium were extracted while the cell wall was broken, filtered, and the filtrate was collected;

[0066] ②Add 120ml of n-hexane to the filter residue in step ①, shear at 25°C for 10min, filter, and collect the filtrate; add 40ml of n-hexane to the filter residue, shear at 25°C for 10min, filter, and collect the filtrate;

[0067] 3. the filtrate obtained in the combined steps 1. and 2. is concentrated in a vacuum at 50°C to obtain 44.9 g of oleoresin with a total carotenoid content of 5.67%, and the extraction rate is 97.9%;

[0068] 4. Add 300 ml of ethanol to the oleoresin in step 3, stir and wash at 50° C. for 10 minutes, filter to remove the solution, and dry at 40° C. for 2 hours to obtain carotenoid crystals ...

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Abstract

The invention relates to a method for preparing natural carotenoid crystals, especially lycopene and beta-carotene by taking microorganism biomasses as raw materials. The method comprises the following steps of: adding an extraction solvent into Blakeslea trispora mycelium, carrying out cell disruption and filtering, and collecting filter liquor; carrying out vacuum concentration on the filter liquor, and recycling solvent to obtain oleoresin; adding a washing solvent into the oleoresin, and washing and filtering to obtain coarse crystals; and drying the coarse crystals and removing residual solvent, thus obtaining the high-content carotenoid crystals. According to the method provided by the invention, the types of the used solvents are few, the use levels of the used solvents are little, the extraction and purification processes are simple, the yield is high, and the method is suitable for industrial production; and the obtained carotenoid crystal product is high in content and good in quality, and can be widely applied to the fields of functional foods, medicines and the like.

Description

technical field [0001] The invention relates to the field of biochemical engineering, in particular to a process for extracting and purifying carotenoids using microbial biomass as raw materials. Background technique [0002] Carotenoids are a class of polyenes that are widely present in plants, animals, microorganisms and algae and contain yellow to dark red polyenes, which have many functions, such as anti-oxidation, anti-cancer, prevention of night blindness, tinting power. Strong wait. As food additives and nutritional supplements, carotenoids have been recognized by FDA, European Community, WHO and other international organizations. In recent years, it has been widely used in the fields of medicine, food, health care products and cosmetics. [0003] Lycopene and beta-carotene are two very important carotenoids, which have strong antioxidant capacity and coloring function. Plants are one of the important sources for obtaining lycopene and beta-carotene, such as tomato...

Claims

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Application Information

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IPC IPC(8): C07C403/24C07C11/21C07C7/00
Inventor 吴文忠王建华徐维锋张显仁
Owner INNOBIO CORP LTD
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