Preparation method of high sulphate group fucoidan polysaccharide

A technology based on fucoidan and high sulfate ester, which is applied in the field of preparation of fucoidan, can solve the problems of low technological content of products, failure to realize maximum value, low processing degree, etc., achieve good cell wall breaking effect, shorten extraction time, The effect of increasing the leaching rate

Inactive Publication Date: 2018-05-08
DALIAN SHENLAN PEPTIDE TECH R & D CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the brown algae in Dalian generally have problems of low processing level and low technological content of products, which cannot realize its maximum value.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Preparation of fucoidan by ultrafine grinding of wakame spore leaves (1)

[0023] Take the dried wakame sporophyll raw material, and pulverize it into wakame sporophyll superfine powder with a particle size of about 20 μm by ultrafine pulverization equipment, and set aside.

[0024] Weigh 500 g of undaria sporophyll superfine powder into a reaction kettle, add pure water according to the mass ratio of material to water ratio of 1:30, and stir and extract at 70° C. for 4 hours.

[0025] The extract was centrifuged at 5000rpm for 15min, and the supernatant was kept, that is, the extract.

[0026] Concentrate the extract under reduced pressure at 0.1MPa vacuum at 70°C to 1 / 5 of the original volume to obtain a concentrated solution.

[0027] Add trichloroacetic acid to the concentrated solution so that the concentration of trichloroacetic acid in the solution is 3.0 g / mL, stir evenly and let it stand for 12 hours, centrifuge at 5000 rpm for 10 minutes to remove ...

Embodiment 2

[0034] Example 2: Preparation of fucoidan by ultrafine grinding of wakame spore leaves (2)

[0035] Take the dried wakame sporophyll raw material, and pulverize it into wakame sporophyll superfine powder with a particle size of about 20 μm by ultrafine pulverization equipment, and set aside.

[0036] Weigh 500 g of undaria sporophyll superfine powder into a reaction kettle, add pure water according to the mass ratio of material to water ratio of 1:30, and stir and extract at 80° C. for 6 h.

[0037] The extract was centrifuged at 5000rpm for 15min, and the supernatant was kept, that is, the extract.

[0038] Concentrate the extract under reduced pressure at 0.1MPa vacuum and 70°C to 1 / 10 of the original volume to obtain a concentrated solution.

[0039] Add trichloroacetic acid to the concentrated solution so that the concentration of trichloroacetic acid in the solution is 3.0 g / mL, stir evenly and let it stand for 12 hours, centrifuge at 5000 rpm for 10 minutes to remove pr...

Embodiment 3

[0046] Example 3: Preparation of fucoidan by ultrafine pulverization of kelp

[0047]Take the dried kelp raw material, pulverize it into wakame sporophyll superfine powder with a particle size of about 20 μm by superfine pulverization equipment, and set aside.

[0048] Weigh 500 g of superfine kelp powder into a reaction kettle, add pure water according to the mass ratio of material to water ratio of 1:30, and stir and extract at 70° C. for 4 hours.

[0049] The extract was centrifuged at 5000rpm for 15min, and the supernatant was kept, that is, the extract.

[0050] Concentrate the extract under reduced pressure at 0.1MPa vacuum at 70°C to 1 / 5 of the original volume to obtain a concentrated solution.

[0051] Add trichloroacetic acid to the concentrated solution so that the concentration of trichloroacetic acid in the solution is 3.0 g / mL, stir evenly and let it stand for 12 hours, centrifuge at 5000 rpm for 10 minutes to remove protein precipitates, and collect the supernat...

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PUM

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Abstract

The invention provides a preparation method of high sulphate group fucoidan polysaccharide, and belongs to the technical field of active polysaccharides. The method solves the problems that in the process of extracting the fucoidan polysaccharide in the prior art, extraction liquid has many impurities; the polysaccharide leaching efficiency is low; the product purity is not high; the polysaccharide decomposing is caused by high temperature, strong acid or strong alkalinity environment in the preparation process; active groups such as sulphate groups are lost, and the like. According to the method, algal is used as a raw material; firstly, the algal is subjected to ultrafine crushing treatment, so that the algal cells are subjected to wall breaking; the polysaccharide leaching rate is increased; the extraction time is reduced; then, a water extraction method is used for extracting the fucoidan polysaccharide; the polysaccharide decomposing and the sulphate group activity loss can be reduced; the natural ingredients and activity of the fucoidan polysaccharide can be greatly remained; finally, the extraction liquid is subjected to protein removal by trichloroacetic acid, sodium alginate impurity removal through ethanol precipitation, and polysaccharide precipitation by ethanol, so that the high-purity and high sulphate group algal polysaccharide is obtained. The purity of the fucoidan polysaccharide prepared by the method can reach 60 to 70 percent; the organic sulfate radical content reaches 20 to 30 percent.

Description

technical field [0001] The invention relates to a method for preparing fucoidan, belonging to the technical field of active polysaccharide preparation. Background technique [0002] Fucoidan, also known as fucoidan, mainly exists in brown algae and some echinoderms. Fucoidan is a special water-soluble polysaccharide combined with sulfate groups, mainly composed of L-fucose, with an average of one sulfate group attached to each monosaccharide, and the most important factor affecting its activity is organic Sulfate and L-fucose content. Studies have shown that fucoidan has various biological activities such as anti-coagulation, anti-tumor, anti-virus, regulating blood lipids, enhancing immune function, anti-aging, protecting body cells, etc. It has great development prospects in cosmetics, functional food and pharmaceutical industries . [0003] The existing fucoidan extraction methods are mainly water extraction, acid extraction and alkali extraction. Generally, the brown ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/04
CPCC08B37/0003C08B37/0063
Inventor 包卫洋张延胜杨大苹王祖哲马普
Owner DALIAN SHENLAN PEPTIDE TECH R & D CO LTD
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