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Method for extracting active polysaccharide from seaweed

A technology of active polysaccharides and seaweeds, applied in the direction of anti-toxins, drug combinations, anti-tumor drugs, etc., can solve the problems that the health effects of seaweed polysaccharides cannot be well guaranteed, the products cannot meet the needs of the society, and the purity and effect cannot be guaranteed. Achieve the effect suitable for large-scale production and application, the extraction method is simple and feasible, and the cell wall breaking effect is good

Inactive Publication Date: 2019-04-12
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, due to the lack of corresponding decomposing enzymes in the human body, the human body cannot directly absorb seaweed polysaccharides by eating seaweed, so it is necessary to extract seaweed polysaccharides from seaweed
However, the current extraction method cannot guarantee the purity and effect of the extraction, and the extraction efficiency is low. The obtained product cannot meet the needs of the society, and there is a problem of poor product quality. At the same time, it cannot be well absorbed by the human body. Finally, seaweed polysaccharide health effects cannot be well guaranteed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for extracting active polysaccharides from seaweed, specifically comprising the following steps:

[0034] S1. Cleaning of seaweed: Take fresh seaweed, first rinse the seaweed in an alkaline water pool for 10 minutes, then remove the seaweed and wash it twice in a clear water pool, each time for 5 minutes, after cleaning, take out the seaweed and place it in slices The seaweed fragments are obtained by shearing in the machine;

[0035] S2. Seaweed pulverization treatment: the chopped seaweed is successively placed in a pulverizer and a superfine pulverizer for pulverization to obtain superfine seaweed powder with a particle size of 10 μm;

[0036] S3. Seaweed degreasing treatment: Degreasing the ultrafine seaweed powder with petroleum ether to obtain defatted seaweed powder; wherein the mass ratio of the ultrafine seaweed powder to petroleum ether is 1:3.5, and degreasing is carried out at a temperature of 50° C. processing, centrifugation after leaching, and d...

Embodiment 2

[0045] A method for extracting active polysaccharides from seaweed, specifically comprising the following steps:

[0046] S1. Cleaning of seaweed: Take fresh seaweed, first rinse the seaweed in an alkaline water pool for 10 minutes, then remove the seaweed and wash it in a clear water pool for 3 times, each time for 5 minutes, after cleaning, take out the seaweed and place it in slices The seaweed fragments are obtained by shearing in the machine;

[0047] S2. Seaweed crushing treatment: the chopped seaweed is successively placed in a pulverizer and an ultrafine pulverizer for pulverization to obtain an ultrafine seaweed powder with a particle size of 15 μm;

[0048] S3. Seaweed degreasing treatment: Degreasing the ultrafine seaweed powder with petroleum ether to obtain defatted seaweed powder; wherein the mass ratio of the ultrafine seaweed powder to petroleum ether is 1:4, and leaching three times at a temperature of 50°C for degreasing processing, centrifugation after leac...

Embodiment 3

[0057] A method for extracting active polysaccharides from seaweed, specifically comprising the following steps:

[0058] S1. Cleaning of seaweed: take fresh seaweed, first rinse the seaweed in an alkaline water pool for 10 minutes, then remove the seaweed and wash it in a clean water pool for 3 times, each time for 7 minutes, after cleaning, take out the seaweed and place it in slices The seaweed fragments are obtained by shearing in the machine;

[0059] S2. Seaweed crushing treatment: the chopped seaweed is successively placed in a pulverizer and a superfine pulverizer for pulverization to obtain superfine seaweed powder with a particle size of 20 μm;

[0060] S3. Seaweed degreasing treatment: Degreasing the ultrafine seaweed powder with petroleum ether to obtain degreased seaweed powder; wherein the mass ratio of the ultrafine seaweed powder to petroleum ether is 1:4.5, and the temperature is 55° C. for 3 times for degreasing processing, centrifugation after leaching, and...

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Abstract

The invention discloses a method for extracting an active polysaccharide from the seaweed, which comprises the steps of seaweed cleaning, seaweed crushing treatment, seaweed degreasing treatment, enzymolysis treatment, super extraction, concentration, protein removal treatment, decolorization treatment, alcohol precipitation and the like. The method for extracting an active polysaccharide from theseaweed comprises the following steps of: firstly carrying out superfine grinding on the seaweed, extracting the polysaccharide of the seaweed by adopting a mode of combining microwave-assisted enzymolysis and super extraction, thereby achieving a good cell wall breaking effect, effectively improving the leaching rate of the polysaccharide and the extraction efficiency is greatly improved, the process route is simple, and the production cost is low. The method for extracting an active polysaccharide from the seaweed is simple and feasible, the extraction conversion rate and the purity of thepolysaccharide are higher, the industrial production is easy with higher safety, and the economic value of the seaweed is greatly improved., The method has mild deproteinization and decolorization processes, shortens the process flow, reduces the energy consumption and the consumption of organic solvents, which is more suitable for large-scale production and application.

Description

technical field [0001] The invention relates to the technical field of seaweed polysaccharide extraction, in particular to a method for extracting active polysaccharides from seaweed. Background technique [0002] In recent years, a large number and variety of marine organisms have been widely used in the fields of food, medicine, cosmetics and biological materials, and the extraction and separation of biologically active substances from marine organisms has become a hot spot in the research of natural products. The most striking thing about marine life is its rich medicinal resources. Scientists have isolated and identified a variety of marine natural active substances from marine life, many of which have biological activities such as antibacterial, antiviral, antitumor and anticoagulant , Laying the foundation for the research and development of new marine drugs. Compared with other biological macromolecules such as proteins, fats, and nucleic acids, carbohydrates are mor...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00A61P39/06A61P39/00
CPCA61P35/00A61P39/00A61P39/06C08B37/0003
Inventor 洪鹏志周春霞刘唤明邓楚津杨萍
Owner GUANGDONG OCEAN UNIVERSITY
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